We have used the technique of fluorescence photobleaching recovery to measure the lateral diffusion coefficients and the mobile fractions of a fluorescent lipid probe, 1-acyl-2-(12-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)aminododecanoyl]) phosphatidylcholine (NBD-PC), and of labeled membrane proteins of human fibroblasts. Values for mobile fractions decrease monotonically with increasing size of th...

Fat mass and obesity-associated protein is an enzyme that oxidatively demethylates DNA. Although there are numerous studies regarding the catalytic function of FTO, the overall existence or absence of FTO on cellular proteome has not been investigated. This study investigated the changes in the soluble proteome of 3T3-L1 cells upon expression of the WT and the mutant (R316Q) FTO proteins. Prote...

Arabidopsis thaliana respiratory burst oxidase homolog D (RbohD) functions as an essential regulator of reactive oxygen species (ROS). However, our understanding of the regulation of RbohD remains limited. By variable-angle total internal reflection fluorescence microscopy, we demonstrate that green fluorescent protein (GFP)-RbohD organizes into dynamic spots at the plasma membrane. These RbohD...

BACKGROUND Multiple sclerosis (MS) is a multifactorial autoimmune disease of the central nervous system with a heterogeneous and unpredictable course. To date there are no prognostic biomarkers even if they would be extremely useful for early patient intervention with personalized therapies. In this context, the analysis of inter-individual differences in cerebrospinal fluid (CSF) proteome may ...

Most empirical work on color constancy is based on simple laboratory models of natural viewing conditions. These typically consist of spots seen against uniform backgrounds or computer simulations of flat surfaces seen under spatially uniform illumination. In this study measurements were made under more natural viewing conditions. Observers used a projection colorimeter to adjust the appearance...

The functional and cost advantages of Internet telephony are evident. By today's technical standards, however, Voice Over IP (VoIP) is still less secure than traditional telephony. At the same time, the hacker scene is constantly looking for new weak spots and developing ingenious methods of attack to gain access to confidential information and penetrate further into the network.

Bowtie aperture antennas are known to generate sub-diffraction limited optical spots in the visible and near-infrared frequencies, which can be applied to many areas. Regular bowtie apertures fabricated by FIB suffer from tapered sidewall and rounded corner, which degrade its optical enhancement and localization. In this work, a new fabrication method is demonstrated to manufacture bowtie apert...

The recent availability of modern big.LITTLE ARM chips featuring heterogeneous processor cores has enabled a practical investigation of the heterogeneous chip concept and its implications for performance and energy. The ODROID XU+E platform produced by Hardkernel integrates hardware power monitors for the individual chip clusters, GPU and Memory. In this paper we investigate the detailed energy...