Caffeine induces loss of calcium and influences the normal development of bone. This study investigated the effects of coffee on bone metabolism in rats by biochemical measurement of calcium, bone densitometry and histometry. Male rats, born of female treated daily with coffee and with coffee intake since born, were anesthetized, subjected to extraction of the upper right incisor, and sacrifice...

OBJECTIVE To evaluate whether treatment with L-arginine influences the healing of skin flaps in rats exposed to nicotine. METHODS 40 male Wistar rats weighing 142.4 ± 10.1 g were separated into four groups: GC: treatment with 7.4 pH phosphate buffer, submitted to skin flap and observation for ten days; GN: exposure to nicotine for four weeks, submitted to skin flap and observation for ten day...

PURPOSE Histological study of vascularization between a cecal pedicle flap and the testicle of Wistar rats. METHODS Fifty-three rats were studied. G1: submitted to celiotomy (a), mobilization of the right testicle (RT) to the abdomen (b), cecal flap suture to the RT (d) and cavity closure. G1: procedures a, b and d and fixation of RT into abdomen. G3: procedures a, b and d, exposition of RT t...

We examined cerebral arteriolar structure and autoregulation of cerebral blood flow (CBF) in control (n = 8), sham-operated (n = 8), pinealectomized (n = 10), and pinealectomized plus melatonin-treated (0.51 +/- 0.01 mg x kg(-1) x day(-1) in drinking water, n = 9) young Wistar rats. The lower limit of CBF autoregulation (LLCBF) was determined by measurement of CBF (in arbitrary units, laser Dop...

Plasma and tissue testosterone concentrations were determined by radioimmunoassay in 12 eight-month-old sexually mature New Zealand White rabbits and evaluated for possible associations with spermatogenic efficiency as well as with volume density and number of Leydig cells. Testicular tissue was processed histologically and histometry was performed in order to quantify germ cells, Sertoli cells...

The intestine of intrauterine growth retarded (IUGR) neonates showed different morphology compared to neonates born with normal body weight (NBW). The aim of the present study was to investigate the ultrastructure and proteomic profile of the gut epithelium in IUGR pig neonates with special attention to the digestive and absorptive function. Intestine tissue samples were investigated in 7-day-o...

The purpose of this study was to evaluate experimental peri-implant breakdown microbiologically, radiographically and histologically. Hydroxyapatite-coated, titanium plasma-sprayed, and titanium alloy surfaces were investigated. Eighty-four implants were placed in 14 beagle dogs. Standardized radiographs and microbiologic samples (DNA) were obtained. Dogs were sacrificed at 3 and 6 months. Unde...

PURPOSE To evaluate bone healing in the extraction socket of the feline maxillary canine tooth after grafting. METHODS Eighteen adult cats were submitted to unilateral extraction of maxillary canine tooth and divided into three groups. In group 1 (n=6), control, the extraction socket was left empty. In group 2 (n=6), the extraction socket was filled with autogenous cancellous bone from the il...

Thyroid tissue removed at partial thyroidectomy from primary thyrotoxicosis patients, after preparation for operation with carbimazole and Lugol's iodine, was studied with the histometric technique. In patients with little or no evidence of autoimmunity before operation, the chance of developing postoperative hypothyroidism was approximately 1 in 2 in those who had volume percentage of epitheli...