Novel HPTLC and stability indicating RP-HPLC methods were developed for simultaneous estimation of Moxifloxacin (MOX) and Dexamethasone (DEX) in ophthalmic dosage form. For HPTLC method, the separation was carried out on HPTLC aluminum plates using acetonitrile:water:ammonia (8:1:0.5 V/V/V) as mobile phase and developed plates were read at 266 nm. The drugs were resolved satisfactorily with Rf ...

Curcumin, the active molecule present in Curcuma longa is known for its antitumour, antioxidant, antiarthritic, anti-amyloid, anti-ischemic, anti-inflammatory activities. In addition, it may also be effective in treating malaria, prevention of cervical cancer, etc. High-performance thin-layer chromatography (HPTLC) method has been employed for determination of curcumin in four varieties of turm...

A repeatable separation of polystyrenes according to MW, from 1920 to 520000 u.m.a, has been obtained under adsorption conditions using a method that comprises the use of Lichrospher HPTLC plates, and a controlled, isocratic elution with a 78:22 (v/v) mixture of cyclohexane (Cy)-tetrahydrofuran (THF). Likewise, UV-densitometric quantification of polystyrenes in mixtures can be achieved, by an i...

The fingerprint profiles of methanol extract and ethyl acetate sub fraction of Azadirachta indica leaves were conducted using preliminary phytochemical screening and high performance thin layer chromatography (HPTLC) analysis. Preliminary phytochemical screening was performed using various biochemical tests. Alkaloid, flavonoid, tannin, saponin and phenol were present in both the extract/subfra...

High Performance Thin Layer Chromatography (HPTLC) is the most simple separation technique available today which gives better precision and accuracy with extreme flexibility for various steps (stationary phase, mobile phase, development technique and detection). We planned our work as to investigate the HPTLC fingerprinting profile of alcoholic extracts of stem bark of F. bengalensis Lin. Colle...

Herbal therapy was the common treatment prescribed by Iranian physicians for wound healing. "Zemad" was the most ancient pharmaceutical dosage form used in Iranian Traditional Medicine (ITM) for skin diseases. In the present research, formulation of a traditionally used Poly Herbal Paste (PHP) for wound healing was performed. Moreover, the fingerprint of the product was prepared by HPTLC method...

AIM Detection of aflatoxin B1 in Livestock compound Feed and feed ingredients by high-performance thin layer chromatography (HPTLC). MATERIALS AND METHODS Chromatography was performed on HPTLC silica gel 60 F 254, aluminum sheets by CAMAG automatic TLC sampler 4, with mobile phase condition chloroform:acetone:water (28:4:0.06). Extraction of aflatoxin B1 from samples was done as per AOAC meth...

The separation of 9 organophosphates (monocrotophos, quinalphos, triazophos, parathion-methyl, isofenphos-methyl, temephos, parathion, phoxim-ethyl, and chlorpyrifos) by high-performance thin-layer chromatography (HPTLC) with automated multiple development was studied. The HPTLC method was developed and validated for analysis of residues of phoxim-ethyl and chlorpyrifos in tea. The sample was e...

Sphingolipids have hydrophilic and hydrophobic properties, different saturation and combination of the oligosaccharide chains and mass homology of species located in a narrow m/z region hampering their recognition. To target sphingolipids for diagnostic purposes, standardized methods for lipid extraction, quali- and quantitative assessments are required. In this study, HPTLC-MALDI MS was adopte...

Identification of 19 molecular species globotriaosylceramides (Gb3) in extracts from a Fabry's plasma patient and healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry online coupling to Mass Spectrometry (MS). Separation carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry twin combining detection the visible at 5...