We investigated the mechanism of interaction of individual L-type channel amino acid residues with dihydropyridines within a dihydropyridine-sensitive a1A subunit (a1ADHP). Mutation of individual residues in repeat III and expression in Xenopus oocytes revealed that Thr is not required for dihydropyridine interaction but that bulky side chains (tyrosine, phenylalanine) in this position sterical...

This study evaluated associations of heat shock proteins (HSP) and an oxidative stress protein, protein deglycase (DJ1), with beef quality tenderness. Samples from the longissimus thoracis (N = 99) were collected pre-rigor (day 0) after 14-d aging. Warner-Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), a trained sensory panel used to determine meat quality. Protein abundanc...

Immunohistochemical demonstration of tumor-associated markers may be valuable as an adjunct to conventional histology for determination of malignancy development in oral mucosal diseases. Accordingly, the present study determined the distribution of some important tumor markers such as heat shock proteins (HSP), fibroblast growth factors (FGF) and transferrin receptors (TF-R) in 22 samples ...

Henöch-Schonlein Purpura (HSP) merupakan kondisi vaskulitis pembuluh darah kecil yang paling sering terjadi pada anak, dengan insidensi 10-20 kasus per 100.000 anak setiap tahunnya. Lebih dari 90% pasien berusia dibawah 10 tahun usia rata-rata 6 tahun. Penyakit ini ditandai lesi kulit spesifik berupa purpura nontrombositopenik, artritis, nyeri abdomen dan perdarahan saluran cerna, serta dapat p...

Mammalian cells constitutively express a cytosolic and nuclear form of heat shock protein (hsp) 70, referred to here as hsp 73. In response to heat shock or other metabolic insults, increased expression of another cytosolic and nuclear form of hsp 70, hsp 72, is observed. The constitutively expressed hsp 73, and stress-inducible hsp 72, are highly related proteins. Still unclear, however, is ex...

In congenital heart disease (CHD), mechanical wall stress by increased pulmonary artery pressure and pulmonary blood flow is believed to play a pivotal role in the pathogenesis of pulmonary plexogenic arteriopathy (PPA). The pathogenesis of this disease that involves significant pulmonary arterial remodelling, is, however, largely unknown. In the systemic circulation, upregulation of HSP-70 and...

Despite the high level of symmetry between PsaA and PsaB polypeptides in Photosystem I, some amino acids pairs are strikingly different, such as PsaA-Gly693 PsaB-Trp673, which located near a cluster 11 water molecules A1A A1B quinones FX iron-sulfur cluster. In this work, we changed PsaB-Trp673 to PsaB-Phe673 Synechocystis sp. PCC 6803. The variant contains ~ 85% wild-type (WT) levels I but is ...