expression of eukaryotic proteins in e. coli often results in their aggregation. proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. this is not achieved in cytoplasmic expression in e. coli because of the absence of disulfide bonds formation. a novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

0

Now culture of manage safety, health and wellbeing in the mining industry needs a scientifically sound new approach to training process for professionals at all levels. Insufficient qualification employees managers occupational has negative impact on injury rate, current management system does not allow instill compliance with requirements their own safety. To improve quality safe work practice...