The greater solubility of human insulin and its possible faster action have led to doubts about whether a sufficiently long acting formulation could be produced to provide a basal supply for diabetics. In a double blind crossover study in 18 diabetics human ultralente insulin was as effective as beef ultralente insulin in controlling basal plasma glucose concentrations (median 5.7 mmol/l (103 m...

Background & Objective: Type1 diabetes is characterized by autoimmune destruction of pancreatic β cells, leading to reduced insulin secretion. Differentiation of mesenchymal stem cells (MSCs) into β-like cells offers new ways of diabetes treatment. MSCs can be insulated from the human umbilical cord tissue and differentiate into insulin-producing cells. Material & Methods: Human um...

The residues A21Asn, B12Val, B16Tyr, B24Phe, B25Phe, B26Tyr and B27Thr, buried in the dimer of insulin, were identified by means of alanine-scanning mutagenesis. The receptor binding activity, in vivo biological potency and self-association properties of the seven single alanine human insulin mutants were determined. Four of the seven single alanine mutants, [B12Ala]human insulin, [B16Ala]human...

Before the discovery of insulin, diabetes was a life-threatening and untreatable illness. It caused by very high blood sugar levels many children died from it. In 1922, two scientists, Frederick Banting Charles Best, treated boy suffering with special extracts cow’s pancreas. The pancreas is an organ found near stomach. treatment lowered boy’s to normal due chemical substance This later named i...

Previous investigations have revealed evidence for species-specificity among various mammalian insulins in immunologic reactions (1-5). For this reason, immunoassay of plasma insulin (610) in any species generally requires that the insulin employed in standard solutions be derived from the same species. Immunoassay of plasma insulin in man has been carried out by comparing the effects of unknow...

Background: Many clinicians recommend low-salt diets for lowering blood pressure but there may be unintended consequences such as worsening insulin resistance.Aim: This paper aimed to find human clinical studies looking at on markers of glucose and insulin.Methods: We reviewed PubMed using the search terms ‘sodium’, ‘insulin’ ‘insulin resistance’ found 23 testing showing negative harms or gluco...

objective: worldwide, diabetes mellitus (dm) is an ever-increasing metabolic disorder. a promising approach to the treatment of dm is the implantation of insulin producing cells (ipc) that have been derived from various stem cells. culture conditions play a pivotal role in the quality and quantity of the differentiated cells. in this experimental study, we have applied various culture condition...