Nanoparticles (NPs) have been widely used in medical and therapeuticapplications. However, their albumin binding and their cytotoxicity assays havenot been well explored. In his study, the interaction of NiO NPs with human serumalbumin (HSA) was explored by circular dichroism (CD) study, molecular dockingand dynamic studies. Afterwards the cytotoxicity of NiO NPs against...

The esterase-like activity of human serum albumin (HSA) toward esters of nicotinic acid was investigated under a variety of conditions such as protein concentration, temperature, pH, ionic strength, nature of buffers, and presence of organic solvents. Initial rate constants of hydrolysis of 18 nicotinates in the presence of 50 microM HSA were measured at pH 7.4 and 37 degrees C. The substrates ...

Human serum albumin (HSA) is a broadly used biomarker for the diagnosis of various diseases such as chronic kidney disease. Here, fluorescent probe TC426 with aggregation-induced emission (AIE) characteristics reported sensitive and specific HSA. This non-emissive in aqueous solution, meanwhile it shows bright fluorescence upon interacting HSA, which makes applicable detecting HSA high signal t...

Determination of intraocular antibody synthesis against certain microorganisms is a diagnostic aid in identifying the causative agent in clinical uveitis. Little is known, however, concerning the kinetics and specificity of antibodies produced during intraocular inflammation. To investigate this subject we induced uveitis in rabbits by injecting small amounts of human serum albumin (HSA) into t...

In recent years, azo dyes have received increasing attention due to their adverse effects on the environment and consumer health. However, interaction mechanism between human serum albumin (HSA) Direct Red 80 (DR80) is still unknown. The results showed that DR80 changed secondary structure of HSA made skeleton loose stretch. addition, quenched endogenous fluorescence by static quenching microen...

The binding of 1-chloro-3-(2-methyl-5-nitro-1H-imidazole-1-yl) propan-2-ol (Ornidazole) to human serum albumin (HSA) was studied by fluorescence and UV-visible spectroscopy. Interaction ornidazole (OR) with HSA identified Stern-Volmer Van’t Hoff equations. constant, Kb the thermodynamic parameters, ∆H, ∆S, ∆G at different temperatures were calculated several Data shows that quenching mechanism ...

The interactions of 3-carboxyphenoxathiin with Bovine Serum Albumin (BSA) and Human Serum Albumin (HSA) have been studied by fluorescence and circular dichroism spectroscopy. The binding of 3-carboxyphenoxathiin quenches the BSA and HSA fluorescence, revealing a 1:1 interaction with a binding constant of about 10(5) M(-1). In addition, according to the synchronous fluorescence spectra of BSA an...

Background: Possibility to trace-label albumin with isotopes results in information concerning its synthesis, breakdown, and distribution in the intra and extra cellular spaces. The iodination of albumin is a widespread procedure used in scientific studies. Bromine not only is more reactive and less expensive than iodine, but bonds more easily with many elements. Therefore, it could be a suitab...

Albumin is known to have neuroprotective effects. The protein has a long half-life circulation, and its effects can therefore persist for a long time to aid in the recovery of brain ischemia. In the present study, we investigated the neuroprotective effects of human serum albumin (HSA) on brain hemodynamics. Albumin is administrated using repeated oral gavage to the rodents. Sprague-Dawley rats...

background glucose is an essential element in the supply of body’s energy. in diseases such as diabetes, glucose increase is associated with disturbance in metabolism. cortisol is an important hormone in the regulation of glucose metabolism, and human serum albumin (hsa) is one of the most important glucose and cortisol transmitters in blood. interaction between albumin and these ligands could ...