Carbon monoxide (CO) is a vasoactive molecule that is generated by vascular cells as a byproduct of heme catabolism and it plays an important physiological role in circulation system. In order to investigate whether exogenous CO can mediate the growth and proliferation of vascular cells, in this study, we used 250 parts per million (ppm) of CO to treat human umbilical artery smooth muscle cell ...

Background: Surface properties of a biomaterial could be critical in determining biomaterial’s biocompatibility due to the fact that the first interactions between the biological environment and artificial materials are most likely occurred at material’s surface. In this study, the surface properties of a new nanocomposite (NC) polymeric material were modified by combining plasma treatment and...

Diabetes mellitus is associated with an increased prevalence of endothelial dysfunction and development of atherosclerotic vascular diseases. We demonstrate here that hyperglycemia results in the expression of adhesion molecules on endothelial cells in vitro. Incubation of human umbilical vein endothelial cells (HUVEC) in a culture medium with 11.0 mM, 16.5 mM and 22.0 mM glucose concentrations...

Scope Pro-inflammatory stimuli such as hyperglycemia and cytokines have been shown to negatively affect endothelial cell functions. The aim of this study is assess the potential quercetin its human metabolites overcome deleterious effects hyperglycemic or inflammatory conditions on vascular endothelium by modulating metabolism. Methods results A metabolomics approach enabled identification quan...

Cunningham, Sonia A., Tuan M. Tran, M. Pia Arrate, Robert Bjercke, and Tommy A. Brock. KDR activation is crucial for VEGF165-mediated Ca21 mobilization in human umbilical vein endothelial cells. Am. J. Physiol. 276 (Cell Physiol. 45): C176–C181, 1999.—We have prepared a polyclonal mouse antibody directed against the first three immunoglobulin-like domains of the kinase insert domain-containing ...

Kalogeris, Theodore J., F. Stephen Laroux, Adam Cockrell, Hiroshi Ichikawa, Naotsuka Okayama, Travis J. Phifer, J. StevenAlexander, and Matthew B. Grisham. Effect of selective proteasome inhibitors on TNF-induced activation of primary and transformed endothelial cells. Am. J. Physiol. 276 (Cell Physiol. 45): C856–C864, 1999.—The objective of this study was to assess the effects of two structura...

Endothelial-selective delivery of therapeutic agents, such as drugs or genes, would provide a useful tool for modifying vascular function in various disease states. A potential molecular target for such delivery is E-selectin, an endothelial-specific cell surface molecule expressed at sites of activation in vivo and inducible in cultured human umbilical vein endothelial cells (HUVEC) by treatme...

Tumor cells have evolved effective strategies to escape the host immune response. The objective of this study was to determine whether tumor cells can condition endothelial cells in a specific manner to prevent subsequent adhesion of polymorphonuclear neutrophils (PMNs) and/or peripheral blood lymphocytes (PBLs). Human umbilical vein endothelial cells (HUVECs) and UKF-NB-4 neuroblastoma tumor c...

The phenotype of endothelial cells (ECs) is specific to the vascular bed from which they originate. To examine how mechanical forces alter the phenotype of different ECs, we compared the effects of cyclic strain and motion control on reactive oxygen species (ROS) production and metabolism and cell adhesion molecule expression in human umbilical vein endothelial cells (HUVEC) vs. human aortic en...

Prekallikrein (PK) activation on human umbilical endothelial cells (HUVEC) presumably leads to bradykinin liberation. On HUVEC, PK activation requires the presence of cell-bound high-molecular-weight kininogen (HK) and Zn(2+). We examined the Zn(2+) requirement for HK binding to and the consequences of PK activation on endothelial cells. Optimal HK binding (14 pmol/10(6) HUVEC) is seen with no ...