The mechanism underlying the action of lignocellulolytic enzymes in biodegradation of lignocellulosic biomass remains unclear; hence, it is crucial to investigate enzymatic interactions involved in the process. In this study, degradation of corn cob by Sporothrix carnis and involvement of lignocellulolytic enzymes in biodegradation were investigated over 240 h cultivation period. About 60% degr...

The microbial conversion of lignocellulosic biomass for biofuel production represents a renewable alternative to fossil fuels. However, the discovery of new microbial enzymes with high activity is critical for improving biomass conversion processes. While attempts to identify superior lignocellulose-degrading enzymes have focused predominantly on the animal gut, biomass-degrading communities in...

Previous communications from this laboratory delineated the events associated with the in vitro cultivation of unstimulated mouse mononuclear phagocytes (1, 2). These processes included a progressive increase in lysosome-like cytoplasmic granules. In addition, it was shown that two inhibitors of protein synthesis blocked the formation of both granules and hydrolytic enzymes. These data as well ...

A histochemical study of hydrolytic enzymes such as acid phosphatase and beta-glucuronidase was made on cultured brain tumor cells. Well-differentiated astrocytic cells, oligodendroglioma cells, and medulloblastoma cells showed low activities. Migrating cells and macrophage-like cells showed intense activities. Meningioma cells and schwannoma cells showed relatively intense activities at the ea...

Aspergillus oryzae koji plays a crucial role in fermented food products due to the hydrolytic activities of secreted enzymes. In the present study, we performed a comparative secretome analysis of the industrial strain of Aspergillus oryzae 3.042 and its spontaneous mutantZJGS-LZ-21. One hundred and fifty two (152) differential protein spots were excised (p<0.05), and 25 proteins were identifie...