نتایج جستجو برای: hydrophobic interaction chromatography

تعداد نتایج: 692711  

Journal: :Journal of chromatography. A 2012
Karla Mayolo-Deloisa Ma Elena Lienqueo Barbara Andrews Marco Rito-Palomares Juan A Asenjo

The chromatographic methods used for the purification of PEGylated proteins are mainly Size Exclusion (SEC) and Ion Exchange Chromatography (IEX). Although the PEGylation affects the protein hydrophobicity, Hydrophobic Interaction Chromatography (HIC) has not been extensively applied for the separation of these proteins. Purification of monoPEGylated Ribonuclease A (RNase A) using HIC is studie...

Journal: :Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2016
Martyna Baca Jelle De Vos Gilles Bruylants Kristin Bartik Xiaodong Liu Ken Cook Sebastiaan Eeltink

The effect of different kosmotropic/chaotropic salt systems on retention characteristics of intact proteins has been examined in hydrophobic interaction chromatography (HIC). The performance was assessed using different column chemistries, i.e., polyalkylamide, alkylamine incorporating hydrophobic moieties, and a butyl chemistry. Selectivity in HIC is mainly governed by the salt concentration a...

Journal: :iranian journal of chemistry and chemical engineering (ijcce) 2002
majid soleimani mohammad nabi sarbolouki

the search for new stationary phases has been one of the predominant concerns in high performance liquid chromatography (hplc) in order to achieve better resolutions, longer column lives, and reduce the time of analysis. a chromatographic packing for separation of underivatized amino acids (aas) were prepared by dynamically coating 2-amino tetraphenyl prophyrin (atpp) on a c-18 reversed-phase p...

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