نتایج جستجو برای: ic rt lamp
تعداد نتایج: 96857 فیلتر نتایج به سال:
BACKGROUND The devastating viral disease of small ruminants namely Peste des petits ruminants (PPR) declared as target for "Global Eradication" in 2015 by the Food and Agriculture Organization (FAO) and the World Organization for Animal Health (OIE). For a successful eradication campaign, molecular diagnostic tools are preferred for their specificity, efficacy and robustness to compliment proph...
چکیده باقلا با نام علمی vicia faba l. یکی از حبوبات عمده در ایران است. ویروس موزاییک زرد لوبیا (bean yellow mosaic virus) در بسیاری از کشورها از مزارع باقلا جداسازی شده است. برای ردیابی و شناسایی آن در مزارع باقلای شهرستان جیرفت، استان کرمان، در سال زراعی 1390، 240 نمونهی برگی دارای علایم موزاییک خفیف تا شدید، کوچکی و بدشکلی برگها و همچنین تعدادی نمونه فاقد علایم ویروسی و 15 نمونه علف هر...
این تحقیق به منظور تعیین واکنش ژنوتیپ های لوبیا نسبت به بیماری ویروس موزائیک نکروز لوبیا(bcmnv) انجام شد. بیست و پنج ژنوتیپ لوبیا تهیه شده از ایستگاه ملی تحقیقات لوبیای خمین در قالب طرح بلوک های کامل تصادفی در سه تکرار در گلخانه ویروس شناسی گروه گیاه پزشکی دانشکده علوم و مهندسی کشاورزی دانشگاه تهران در سال 1388 کشت و در مرحله دو برگی توسط این ویروس آلوده سازی شدند. ارزیابی گیاهان پس از 21 روز ب...
abstract: potato virus y a type species of the genus potyvirus infects several crops in the family solanaceae. pvy isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. the result of elisa, immunoblot electrophoresis and immuno capture reverse transcription polymerase chain reaction (ic-rt-pcr) indicated that pepper isol...
Speed is paramount in the diagnosis of highly infectious diseases, such as foot-and-mouth disease (FMD), as well as for emerging diseases; however, simplicity is required if a test is to be deployed in the field. Recent developments in molecular biology have enabled the specific detection of FMD virus (FMDV) by reverse-transcription loop-mediated isothermal amplification (RT-LAMP), real-time re...
Crimean-Congo hemorrhagic fever (CCHF) virus (CCHFV) activity has been detected in Kordufan region of the Sudan in 2008 with high case-fatality rates in villages and rural hospitals in the region. Therefore, in the present study, a reverse transcription (RT) loop-mediated isothermal amplification (RT-LAMP) assay was developed and compared to nested RT-PCR for rapid detection of CCHFV targeting ...
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swi...
Ebola virus (EBOV) can lead to severe hemorrhagic fever with a high risk of death in humans and other primates. To guide treatment and prevent spread of the viral infection, a rapid and sensitive detection method is required for clinical samples. Here, we described and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method to detect Zaire ebolavirus using the ...
Here, we describe a uracil-DNA glycosylase (UNG)-treated reverse transcription loop-mediated isothermal amplification (uRT-LAMP) for the visual detection of all subtypes of avian influenza A virus (AIV). The uRT-LAMP assay can prevent unwanted amplification by carryover contamination of the previously amplified DNA, although the detection limit of the uRT-LAMP assay is 10-fold lower than that o...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید