Elevated IgE levels and increased IgE sensitization to allergens are central features of allergic asthma. IgE binds to the high affinity IgE receptor FcεRI on mast cells , basophils , and dendritic cells , and mediates the activation of these cells upon antigeninduced crosslinking of IgE-bound FcεRI. FcεRI activation proceeds through a network of signaling molecules and adaptor proteins and is ...

Elevated IgE levels and increased IgE sensitization to allergens are central features of allergic asthma. IgE binds to the high affinity IgE receptor FcεRI on mast cells , basophils , and dendritic cells , and mediates the activation of these cells upon antigeninduced crosslinking of IgE-bound FcεRI. FcεRI activation proceeds through a network of signaling molecules and adaptor proteins and is ...

Asthma is a disease characterized by spontaneous contraction of the airways in response to a wide variety of endogenous and exogenous stimuli. Many asthma models are used to mimic the human asthma model in the literature. In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and...

An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE-dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutr...

We demonstrate using primary mast cell cultures derived from wild-type and CD45-deficient mice that mast cell triggering through the high-affinity immunoglobulin E (IgE) receptor requires the cell surface tyrosine phosphatase CD45. Unlike wild-type cells, cross-linking of surface-bound IgE in mast cells deficient in CD45 does not induce degranulation. Degranulation in these mutant cells does oc...

The novel cytokine IL-31 and its receptor have been shown to play a central role in bridging the immune system with neurons, epithelial surfaces connective tissues. Although increasing evidence has demonstrated their inflammation, involvement expansion of distinct leukocyte subsets is not fully understood. Detailed analyses Il31-transgenic (Eμ-Lck) mice indicated that next induction pruritus sk...

The high affinity receptor for IgE on rat basophilic leukemia (RBL) cells mediates antigentriggered cellular degranulation. Polyethylene glycolinduced membrane fusion methods were used to introduce exogenous IgE receptors into living RBL cells, and these were tested for normal activities. In cellcell fusion experiments, RBL cells with fluoresceinlabeled rat IgE bound to receptors and containing...

Background Treatment of Chronic Idiopathic Urticaria (CIU) may be challenging, as patients can be refractory to various treatments. This may be due to an autoimmune mechanism in its pathogenesis. CIU has been shown to be associated with autoantibodies to IgE or IgE receptor and with thyroid autoimmunity [1,2]. Treatments targeting this autoimmune pathogenesis are now being used, such as intrave...

To map precisely the binding site of the high affinity receptor (FcεRI) on IgE we have constructed and expressed recombinant human and mouse IgE genes with anti-NIP specificity. Various mutated and chi-meric molecules thus prepared were studied for their ability to bind to rat mast cells or transfected fibro-blasts expressing human FcεRI α chain. To avoid destruction of the FcεRI binding site d...

The novel cytokine IL-31 and its receptor have been shown to play a central role in bridging the immune system with neurons, epithelial surfaces connective tissues. Although increasing evidence has demonstrated their inflammation, involvement expansion of distinct leukocyte subsets is not fully understood. Detailed analyses Il31-transgenic (Eμ-Lck) mice indicated that next induction pruritus sk...