DNAimage cytometry is a relatively new technique for densitometric measurement of nuclear DNA content, which has only rarely been used in botany and thus no methodological standards exist for this method to be applied to the measurement of plant material. In the present paper we address several problems related to standardisation of DNA image cytometry, such as stability of the measuring system...

Late diagnosis resulting in late treatment and locoregional failure after surgery are the main causes of death in patients with oral squamous cell carcinomas (SCCs). Actually, exfoliative cytology is increasingly used for early detection of oral cancer and has been the subject of intense research over the last five years. Significant advances have been made both in relation to screening and eva...

A simple, yet powerful magnetic-levitation-based device is reported for real-time, label-free separation, as well as high-resolution monitoring of cell populations based on their unique magnetic and density signatures. This method allows a wide variety of cellular processes to be studied, accompanied by transient or permanent changes in cells' fundamental characteristics as a biological material.

Lindblad J. 2003. Development of Algorithms for Digital Image Cytometry. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 789. 67 pp. Uppsala. ISBN 91-554-5497-6. This thesis presents work in digital image cytometry applied to fluorescence microscope images of cultivated cells. Focus has been on the development and compi...

Retinopathic changes are common to many ocular diseases and if detected early, much of the change caused by various injuries can be prevented or in some cases reversed. However, detection and quantification of these changes currently requires tedious invasive and manual examination of retinal wholemount images. To remedy the quantitative limitations, we have designed a software system to automa...

This paper describes a simple, inexpensive multispectral imaging system for image cytometry applications. The system is based on an acousto-optical tunable filter (AOTF), a monochrome CCD camera, and a research-grade fluorescence microscope. The selected TeO2 AOTF has a 10x10 mm 2 entrance aperture and operates within the spectral range of 447–750 nm. The bandpass of the filter varies between 1...

BACKGROUND We coin two terms: First, chemical cytometry describes the use of high-sensitivity chemical analysis techniques to study single cells. Second, metabolic cytometry is a form of chemical cytometry that monitors a cascade of biosynthetic and biodegradation products generated in a single cell. In this paper, we describe the combination of metabolic cytometry with image cytometry to corre...