BACKGROUND Hard exudates (HEs) are the classical sign of diabetic retinopathy (DR) which is one of the leading causes of blindness, especially in developing countries. Accordingly, disease screening involves examining HEs qualitatively using fundus camera. However, for monitoring the treatment response, quantification of HEs becomes crucial and hence clinicians now seek to measure the area of H...

Digital-darkfield analysis involves the spatial mapping of selected frequencies across a digitized image, much as analog (e.g. electron-optical) darkfield-imaging allows one to locate crystals responsible for a given diffraction spot. This analysis involves a type of image decomposition which, like wavelets, is intermediate between direct and reciprocal (spatial-frequency) space [1]. Digital-da...

BACKGROUND The reproducibility of measurements on radiographs is influenced by the techniques by which the images as well as the measurements are obtained. Thus, bias resulting from errors in the image and/or image examinations at two points in time may result in wrongful registrations of true biological or pathological changes. The aim of the present study was to propose and evaluate an indire...

PURPOSE To evaluate the reproducibility of ImageJ software in analyzing the color of the optic disc. METHODS One hundred twelve normal participants (56 males and 56 females) were enrolled in this study. The image of the optic disc was taken using Kowa digital disc photo-graphy, and the gray scales of the nasal rim (NR), brightest cupping center (BCC) and largest inferior retinal vein (IRV) we...

introduction: the limited resolution of the imaging system causes partial volume effects (pves). these results in spreading of image counts to the neighboring pixels. this phenomenon is called spill-out effect. this study aimed at quantifying pves using imagej. methods:technetium-99m solution of concentration of 74 kbq/ml was filled into spheres a, b and c of diameters: 26 mm, 20 mm and 16 mm r...

INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.

ImageFlow is a plugin that allows the generation and execution of macros in ImageJ based on the visual programming paradigm. Image processing steps are described by connected nodes on a workspace. Specifying image-processing algorithms corresponds to visually editing block diagrams (the workflow of successive processing steps). This abstraction makes it very flexible and easy to save, restore a...