OBJECTIVE To assess the efficiency of a "freeze-all" embryo strategy after immature oocyte retrieval (OR) and in vitro maturation (IVM) in patients with polycystic ovary syndrome (PCOS). DESIGN Retrospective case series. SETTINGS University-based tertiary referral center. PATIENT(S) Seventy-nine consecutive PCOS patients undergoing IVM followed by vitrified-warmed embryo transfer (ET) ove...

Vitrifying oocytes is a potentially valuable means of preserving the female germ line, but significantly compromises oocyte developmental competence. This study examined the hypothesis that the cumulus complex protects the oocyte during vitrification. Vitrified-warmed immature cumulus oocyte complexes (COCs) were labelled with a plasma membrane impermeant DNA marker (ethidium homodimer-1) to ex...

OBJECTIVE We evaluated the fertilization potential of immature oocytes obtained from controlled ovarian hyperstimulation cycles of patients undergoing ICSI. METHODS We retrospectively analyzed 463 ICSI cycles containing at least one immature oocyte at oocyte denudation. ICSI was performed on mature oocytes at oocyte denudation (metaphase-II [MII] oocytes) and the oocytes that extruded the fir...

Empty follicle syndrome (EFS) is a condition in which no oocytes are obtained after an apparently successful ovarian stimulation. Genuine EFS (GEFS) is differentiated from false EFS by an optimal level of human chorionic gonadotropin on the day of oocyte retrieval. Some believe that GEFS does not exist and that it is only a reflection of the margin of error attendant upon the procedure of oocyt...

Abstract Background The gene expression of anti-Mullerian hormone receptor type 2 ( AMHR2 ) and follicle stimulating (FSHR) in cumulus cells (CCs) isolated from mature immature oocytes was studied as a possible non-invasive approach for determining oocyte maturity quality. CCs 100 infertile women with different etiologic factors were subdivided into control (CCs MII) case GV) group. Q-PCR used ...

Improved oncological treatments permit increased survival rates, although cancer patients remain at risk of losing ovarian function. An attractive option for fertility preservation includes the use of immature oocytes, a strategy which can occur on a rapid timeline and without hormonal stimulation. As a result, cancer therapy can proceed promptly even in patients with hormone-sensitive tumors. ...

Background: 15% of oocytes collected in ART cycles are immature. These oocytes may be matured following IVM program. It is possible to cryopreserve the immature oocytes for further use in ART after application of IVM. The aim was to determine the maturation rate, morphology and viability of human immature oocytes after fresh IVM and vitrified-IVM program. Materials and Methods: 63 women who und...

The serpin peptidase inhibitor, clade E, member 2 (SERPINE2) inhibits urokinase-type plasminogen activator (PLAU) and tissue-type plasminogen activator. Higher SERPINE2 expression levels were detected in cumulus cells of human immature oocytes than in those of mature oocytes. The objective of this study was to evaluate whether high SERPINE2 levels in cumulus cells are associated with oocyte imm...