This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2'-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable ...

The kinetic parameters of both glucose isomerization to fructose and immobilized glucose isomerase (GI) inactivation calculated under different conditions are compared and discussed. Utilizing these figures, the possibility of generalizing a linear model, previously proposed for the kinetics of glucose isomerization by immobilized glucose isomerase, is investigated, so as to apply them to whole...

Whole-cell luminescent bioreporter sensors based on immobilized recombinant Escherichia coli are described and evaluated. The sensors were prepared by glutaraldehyde-anchoring of nonspecific anti-E. coli antibodies on aminosylilated gold or silica glass surfaces with subsequent attachment of the probe bacteria. We demonstrate the generality of the concept by attachment of several E. coli strain...

BACKGROUND Current gene expression analysis relies on the assumption that the isolated RNA represents all species of mRNA in proportions equal to those in the original materials. No system is available for absolute quantification of mRNA. METHODS We applied whole blood to 96-well filterplates to trap leukocytes. Lysis buffer containing cocktails of specific reverse primers and known concentra...

objective(s): enzyme immobilization via nanoparticles is perfectly compatible against the other chemical or biological approximate to improve enzyme functions and stability. in this study lactoperoxidase was immobilized onto silica-coated magnetite nanoparticles to improve enzyme properties in the presence of cadmium chloride as an inhibitor. materials and methods:  the process consists of the ...

AaCel9A [β-1,4-endoglucanase, (E.C:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. The properties of the immobilized AaCel9A were investigated by determining the optimum pH and optimum temperature for activity, thermal stability, and kinetic parameters. The immobilization process shifted the enzyme’s optimum temperature from 65 °C for the fr...

the abilities of silybum marianum cell culture to biotransform benzaldehyde to benzyl alcohol in three culture systems were compared. callus cultures of silybum marianum were established from seedlings, and healthy suspensions and immobilized cultures grown on the murashige and skoog medium. s. marianum cells were immobilized in both agar beads and fiber cotton matrixes. benzaldehyde was fed to...

As part of a research program aimed at producing biodiesel fuel from plant oils enzymatically cells of Rhizopus oryzae (R. oryzae) IFO4697 (with a 1,3-positional specificity lipase) immobilized within biomass support particles (BSPs) were investigated for the methanolysis of soybean oil. The R. oryzae cells easily became immobilized within the BSPs during batch operation. To enhance the methano...

The immobilization of erythrocyte as the whole cell without hemolysis was studied. It found that erythrocyte could be treated and immobilized stably by radiation polymerization of specific monomers having high viscous and long oxyethylene units chain such as methoxypolyethyleneglycol methacrylate (M-23G) and polyethyleneglycol dimethacrylate (14G). Irradiation dose without hemolysis was limited...

Detection of tumor markers is important for cancer diagnosis. Field-effect transistors (FETs) are a promising method for the label-free detection of trace amounts of biomolecules. However, detection of electrically charged proteins using antibody-immobilized FETs is limited by ionic screening by the large probe molecules adsorbed to the transistor gate surface, reducing sensor responsiveness. H...