The paper describes development of a sol-gel based immunoaffinity method for the steroid hormone levonorgestrel (LNG) and the effects of changes in the sol-gel matrix format on the activity of the entrapped antibodies (Abs) and on matrix structure. The best sol-gel format for Ab entrapment was found to be a tetramethoxysilane (TMOS) based matrix at a TMOS:water ratio of 1:8, containing 10% poly...

Detection of distinct cell surface markers has brought up remarkable improvements not only in diagnosis and classification of leukemic diseases, but also has gained immediate clinical relevance concerning therapeutic aspects. Especially. such markers may give information about the antigenic phenotype reflecting the origin of distinct leukemic cells from different stem cell compartments and thei...

La prise en charge des anémies hémolytiques auto-immunes (AHAI) est relativement bien standardisée à la phase aiguë et repose, dans le cadre AHAI anticorps chauds, sur corticothérapie première intention plus ou moins associée un recours précoce au rituximab. Les formes sévères d’AHAI conduisant les patients soins intensifs ne sont pas exceptionnelles associées une mortalité importante [1] , [2]...

Les polymyosites sont des maladies inflammatoires des muscles striés, d'étiologie inconnue. Le déficit musculaire, qui se résume généralement à une fatigabilité, évolue de façon bilatérale, symétrique et non sélective avec prédominance sur les muscles proximaux. L'intensité de la faiblesse musculaire est variable d'un sujet à un autre, de la simple gêne fonctionnelle à un état grabataire. Nous ...

OBJECTIVE To analyze the immune status in situ of tonsils of patients with recurrent tonsillitis (RT) and idiopathic tonsillar hypertrophy (ITH) with the aim to discuss the indications of tonsillectomy (TE) and tonsillotomy (TT) in young children. METHODS The histological and immunohistological study of tonsillar tissue of RT and ITH in correlation with immunological parameters in peripheral ...

The genomic region of Grapevine fanleaf virus (GFLV) encoding the movement protein (MP) was cloned into pET21a and transformed into Escherichia coli strain BL21 (DE3) to express the protein. Induction was made with a wide range of isopropyl-β-D-thiogalactopyranoside (IPTG) concentrations (1, 1.5, and 2 mM) each for duration of 4, 6, or 16 h. However, the highest expression level was achieved wi...

N.M. Wulffraat and MW Heijstek, Department of pediatric rheumatology, UMC Utrecht, The Netherlands In order to develop evidence-based recommendations for vaccination of pediatric patients with auto-immune and/ or auto-inflammatory diseases, a EULAR task force performed a systematic literature review. Available evidence was critically appraised using a customary scoring system for the level of e...

OBJECTIVE Recent data suggest that the low thyroid function syndrome in depression is nonspecific. They also suggest that depression may constitute a risk factor for the development of dementia, especially in atypical patients who have high rates of hypothalamo-pituitary-adrenal axis disorders. This study aimed to search for correlations among Dexamethasone Suppression Test (DST) cortisol level...