An immunohistochemical method using biotinyl tyramine was recently introduced to amplify weak staining signals. Despite its high sensitivity, however, tyramine-based immunostaining has been limited by its increased background staining. In this study, to develop an improved protocol of biotinyl tyramine-based immunohistochemistry minimizing the background staining, we determined which staining s...

• A Zirconium labeled drug was visualized in tumors by Imaging Mass Spectrometry. Time of Flight Secondary Ion Spectrometry (ToF-SIMS) used parallel with immunohistochemistry. ToF-SIMS is a quick and sensitive technique to image zirconium labelled biologics. high-precision complement immunoPET using the same established radiotracers. The human epidermal growth factor receptor 2 (HER2) specific ...

Background & Objective: In vascular (vasculogenic) mimicry (VM), tumoral cells mimic the endothelial cells and form the extracellular matrix-rich tubular networks. It has been proposed that VM is more extensive in aggressive tumors. This study was designed to investigate the rate of VM expression in the stromal cells of invasive ductal carcinoma (IDC) and to find its relationsh...

Background: Retinal G-protein–coupled receptor opsin (RGR) functions as a photoisomerase that convert the all-trans-retinal to 11-cis-retinal in human retinal pigmented epithelium. Our research and others have demonstrated opsin-based UVR phototransduction system existed skin. However, whether RGR is expressed skin has not yet been identified. Objective: To determine expression, distribution su...

Aims—Although positive and negative controls are performed and checked in surgical pathology cases undergoing immunohistochemistry, internal quality control procedures for immunohistochemistry are not well described. This study, comprising a retrospective audit, aims to describe a method of internal quality control for immunohistochemistry. A scoring system that allows comparison between cases ...