The aim of the study was to determine chromosome number and nuclear DNA content an endemic plant, V. scamandri, using 14-week-old plants germinated from seed genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 2,4-D 9-week-old propagated plants, developed 2 0.1 2,4-D. In mitotic counts showed scamandri had 2n = 16. Flow cytometric analys...

an in vitro plant regeneration protocol was successfully established in aphyllorchis montana , a saprophytic achlorophyllous orchid by culturing immature seeds. among the six basal media evaluated for seed germination, bm-tm medium was found to be the best followed by kc medium. after 40 days, all the media turned brown and the growths of the protocorms were arrested. activated charcoal, 1 g/l ...

Taxon Proposed annotation Current Annotation Cactaceae spp. (#4) and Orchidaceae spp. (#8) in Appendix II, and all taxa annotated with #1 Designates all parts and derivatives, except: a) seeds, spores and pollen (including pollinia), except seeds of Mexican Cactaceae spp. originating in Mexico; b) seedling or tissue cultures obtained in vitro, in solid or liquid media, transported in sterile co...

A tissue culture technique for micropropagation of chia (Salvia hispanica L.) was established. High percentage of seed germination (100%) was recorded on Murashige and Shoog medium enriched with 0.4 mg l gibberellic acid and 10 mg l ascorbic acid after one week of culture. The maximum number of shoots per explant (2.7) gave MS medium with 2 mg l 6-benzylaminopurine after five weeks of culture. ...

Plants propagated by seed do not ensure genetic uniformity and are sometimes infected with diseases. In Vitro micropropagation techniques an alternative method to traditional cloning approaches for producing true-to-type pathogen-free plants. However, due the particularities of in vitro environment, these plants face many challenges, often critical their survival, adapt ex conditions. this cont...