This study aimed to determine gender-based differences in fuel metabolism in response to long-duration exercise. Fuel oxidation and the metabolic response to exercise were compared in men (n = 14) and women (n = 13) during 2 h (40% of maximal O2 uptake) of cycling and 2 h of postexercise recovery. In addition, subjects completed a separate control day on which no exercise was performed. Fuel ox...

The effect of experimentally increased wing loading on the energy cost of flight was examined in cockatiels Nyphicus hollandicus. Five individuals were flown for periods of approximately 2 min, while carrying additional payload mass amounting to between 5 and 20% of unloaded body mass. The energy cost of flight was measured using the 13C-labelled bicarbonate technique, which was also calibrated...

In this review we discuss practical considerations for the assessment of brown adipose tissue in rodent models, focusing on mice. The central aim of the review is to provide a critical appraisal of the utility of specialized techniques for assessing brown adipose tissue function in vivo. We cover several of the most common specialized methods for analysing brown adipose tissue function in vivo,...

Few strong and consistent associations have arisen from observational studies of dietary consumption in relation to chronic disease risk. Measurement error in self-reported dietary assessment may be obscuring many such associations. Attempts to correct for measurement error have mostly used a second self-report assessment in a subset of a study cohort to calibrate the self-report assessment use...

Accurate and sensitive assessments of energy expenditure (EE) in mice changes in the balance of gut microbial species could differentially are essential given their importance to research on the molecular mechanisms of energy homeostasis [1]. EE phenotyping in mice is widely available using state-of-the-art equipment in many laboratories, including those in regional centers in the United States...

TO THE EDITOR: the recent paper by Longo et al. (5) provides an excellent description of the challenges of performing indirect calorimetry in mice. Mice that gain weight or are weight stable in their home cages often lose weight during the indirect calorimetry measurements, indicating that the procedure alters the usual behavior of the animals. To tackle this difficulty, the authors proposed a ...

We compared the accuracy of five objective methods, including two newly developed methods combining accelerometry and activity type recognition (Acti4), against indirect calorimetry, to estimate total energy expenditure (EE) of different activities in semi-standardized settings. Fourteen participants performed a standardized and semi-standardized protocol including seven daily life activity typ...