Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasmaphagocytophilum and Ehrlichiachaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were reco...

BACKGROUND The serological diagnosis of avian influenza (AI) can be performed using different methods, yet the haemagglutination inhibition (HI) test is considered the 'gold standard' for AI antibody subtyping. Although alternative diagnostic assays have been developed, in most cases, their accuracy has been evaluated in comparison with HI test results, whose performance for poultry has not bee...

A seasonal distribution was observed for rotaviruses in sewage by using indirect immunofluorescence. Levels were low from May through September and generally higher during winter and spring. In contrast, no seasonal pattern was observed for total enteroviruses. Limitations of the indirect immunofluorescence assay and enzyme immunoassay for environmental samples are discussed.

Twenty-five human dental periapical lesions were obtained. The lesions were frozen, sectioned, and examined for the presence of immune complexes by employing an anticomplement immunofluorescence (ACIF) technique. Twenty-three of the periapical specimens were positive for antigen-antibody complexes. No staining was noted in the two lesions that were histologically diagnosed as periapical scar ti...

The results of antinuclear antibody tests using the indirect immunofluorescence technique may be reported as a description of the pattern and the intensity of fluorescence obtained at a certain dilution. If quantitative results are required titration is necessary. Such titrations may vary greatly between different laboratories. The present study involving 26 laboratories shows an improvement of...

Conventional tube cell culture was compared with a 2 day and 5 day spin-amplified shell vial indirect immunofluorescence assay for the detection of mumps virus in swabs from the area of Stensen's duct. The sensitivity and specificity of the shell vial assay were 95.9 and 100%, respectively. The shell vial detected 66.3% of the positive cultures within 2 days of inoculation while the first posit...

A test for farmer's lung is described employing the indirect fluorescent antibody technique. The test compared very favourably with standard precipitin tests, detecting 88% of a series of positive cases, as opposed to 38% detected by a precipitin test. The fluorescent antibody test, in addition, introduced some measure of quantitation of the serological response in the disease.

An outbreak of an epidemic disease occurred in a specified-pathogen-free (SPF) breeding colony of rats. The clinical signs and the post-mortem findings were characteristic for Tyzzer's disease. The causative agent, Bacillus piliformis, was demonstrated microscopically in ileum, liver and myocardium, and transmitted to mice where its pathogenicity appeared to be similar to that of another strain...

The prevalence of Taiwan monkeys with antibodies to Epstein-Barr virus, as detected by the indirect immunofluorescence technique, was lower than that of healthy persons in Taiwan.

An immunohistochemical procedure for the detection of immunoglobulin G adherent to platelets is described. The peroxidase anti-peroxidase method is used to detect antibody activity directed against platelets from normal donors in the sera from 305 individuals. These subjects were divided into three groups: group 1, patients referred for tissue typing; group 2, healthy normal females; group 3, h...