background fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. they are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species. objectives the present study aims to establish and characterize a novel fish cell line from muscle tissue of wallago attu ca...

In efforts to prepare more potent and sensitive viral serological antigens, several aspects of the production of antigens from infected cell cultures were studied. Antigens derived from whole, infected culture material and from the cellular and fluid phases were compared. Freezing and thawing, sonication, and alkaline buffer extraction were compared for effectiveness in releasing antigen from h...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

statement of problem: many studies have indicated that genetic disturbances are common findings in patients with oral squamous cell carcinoma (oscc). identification of these changes can be helpful in diagnostic procedures of these tumors. purpose: the aim of this study was to appraise the chromosomal disorders in blood and tissue patients with oscc. methods and materials: in this descriptive st...

objective: to study the mechanisms involved in amyloid formation processes, we made a mammalian cell culture model of amylin aggregation and characterized its properties. materials and methods: amyloid fibrils were extracted from cho cells and binding affinity to thioflavin t and congo red was investigated. then the apple-green birefringence of extracted fibrils was detected under polarized li...

AIM Objective of the present study was to isolate bovine herpes virus Type 1 (BHV-1) from semen of infected bull and to adapt it onto embryonated eggs and Madin-Darby bovine kidney (MDBK) cell line. Further, the virus was identified by agar gel immunodiffusion (AGID) test. MATERIALS AND METHODS Semen samples were collected from five BHV-1 positive bulls previously confirmed for the presence o...

Abstract The structural integrity of the host red blood cell (RBC) is crucial for propagation Plasmodium spp . during disease-causing stage malaria infection. To assess stability vivax -infected reticulocytes, we developed a flow cytometry-based assay to measure osmotic within characteristically heterogeneous reticulocyte and P. samples. We find that erythroid decreases erythropoiesis maturatio...

BACKGROUND Hepatic steatosis is a risk factor for both liver disease progression and an impaired response to interferon alpha (IFN-α)-based combination therapy in chronic hepatitis C virus (HCV) infection. Previously, we reported that free fatty acid (FFA)-treated HCV cell culture induces hepatocellular steatosis and impairs the expression of interferon alpha receptor-1 (IFNAR1), which is why t...