Aberrant methylation has been shown to trigger the inactivation of tumor suppressor genes during tumorigenesis. MicroRNAs (miRNAs) have been found deregulated in human colorectal cancer (CRC), and some of them may function as tumor suppressor genes. Here, we investigated CpG island promoter hypermethylation as a potential mechanism underlying miRNA disruption and identifed methylation-sensitive...

BACKGROUND The Cryptococcus neoformans polysaccharide capsule is a well-characterized virulence factor with immunomodulatory properties. The organism and/or shed capsule is postulated to raise intracranial pressure (ICP) in cryptococcal meningitis (CM) by mechanical obstruction of cerebrospinal fluid (CSF) outflow. Little is known regarding capsule phenotype in human cryptococcosis. We investig...

Chk1 is an evolutionarily conserved protein kinase that regulates cell cycle progression in response to checkpoint activation. In this study, we demonstrated that agents that block DNA replication or cause certain forms of DNA damage induce the phosphorylation of human Chk1. The phosphorylated form of Chk1 possessed higher intrinsic protein kinase activity and eluted more quickly on gel filtrat...

Pseudomonas plecoglossicida is a gram-negative pathogenic bacterium that causes visceral white spot disease in several marine and aquaculture fish species, resulting high mortality severe financial loss. Based on previous RNA sequencing (RNA-seq), fliK gene expression significantly up-regulated P. during infection, indicating may contribute to its bacterial pathogenicity. To investigate the rol...

Background: Intrahepatic cholestasis of pregnancy (ICP) is a disorder specifically associated with pregnancy. Recent evidence suggests that the T helper 17 (Th17) cell population related to maternal and foetal immune imbalance ICP. However, there has been insufficient attention paid potential roles signal transducer activator transcription 3 (STAT3) RAR-related orphan receptor gamma (RORγt) in ...

this study was aimed to detect anti-toxoplasma gondii antibodies in cats infected with rh strain of t. gondii by using an immunoblotting method. for this, cats were experimentally infected using tachyzoites harvested from vero cell cultures. tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to pvdf membranes. an immunoblotting was performed using these memb...

BACKGROUND Safety assessment of nanoparticles (NPs) requires techniques that are suitable to quantify tissue and cellular uptake of NPs. The most commonly applied techniques for this purpose are based on inductively coupled plasma mass spectrometry (ICP-MS). Here we apply and compare three different ICP-MS methods to investigate the cellular uptake of TiO2 (diameter 7 or 20 nm, respectively) an...

Human BSEP (ABCB11) mutations are the molecular basis for at least three clinical forms of liver disease, progressive familial intrahepatic cholestasis type 2 (PFIC2), benign recurrent intrahepatic cholestasis type 2 (BRIC2), and intrahepatic cholestasis of pregnancy (ICP). To better understand the pathobiology of these disease phenotypes, we hypothesized that different mutations may cause sign...

Abstract BACKGROUND: In vitroexperiments in which HIV-infected CD4 +T cells are co-cultured with natural killer (NK) yield incomplete elimination of the infected cells. We therefore hypothesize that cell-intrinsic pathways differentially regulated to mediate resistance NK cell-mediated killing. METHODS: Mock-infected and HIV-89.6-infected from 6 HIV −donors were overnight +/− autologous Followi...