An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...

In order to evaluate the tuber fresh weight and some physiological attributes of Agria potato cultivar in response to water deficit stress and mycorrhiza inoculation, a field trial was conducted as a split plot scheme based on randomized complete block design with three replications at the research site of Islamic Azad University, Tabriz Branch in 2012. Four water levels including: irrigation a...

To study the effect of chemical and biological fertilizers on growth and essential oil content of lavender (Lavandula angustifolia Mill.), the present work was conducted in 2012-2013 at the experimental field of Alborz Research Station of the Research Institute of Forests and Rangelands, Karaj, Iran. The experiment was conducted in factorial in the form of a randomized complete block design wit...

Methods for diagnosis of viral infection have progressed rapidly during the past two to three decades from animal inoculation to computer automation. Virus isolation, however, still remains the "gold standard." With the availability of antiviral agents, physicians now demand accurate laboratory diagnosis of their patients' illnesses in order to give proper treatment. Discovery of unknown viral ...

In connection with certain investigations being carried out in this laboratory, it was found necessary to work with sixto twelve-hour cultures of bacteria in fluid culture media. The apparatus described below was devised to obviate the need of either coming to the laboratory late in the evening to inoculate cultures for the following day's work or of wasting time waiting for cultures to grow ou...

The reasons for using sterilized pus in the place of autogenous or other vaccines are : 1. The great simplicity and cheapness. 2. The pus is truly autogenous, this is not so with vaccines prepared from organisms grown on artificial media; for the organism is changed by such growth. 3. Besides the bacteria, pus contains toxins, and antibodies both extremely useful for therapeutic inoculation. Th...

INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350 Jouy-en-Josas, France; INRA, UR444 Laboratoire de génétique cellulaire, F-31320 CastanetTolosan, France; INRA, UMR1286; CNRS, UMR5226, Laboratoire PsyNuGen, F-33076 Bordeaux, France; INRA, UE967 Génétique expérimentale en productions animales, F-17700 Surgères, France; INRA, UAR2 Services déconcentrés d’appui à la recherche – Poit...

Fungi that occur inside asymptomatic plant tissues are known as fungal endophytes. Different genera of fungal entomopathogens have been reported as naturally occurring fungal endophytes, and it has been shown that it is possible to inoculate plants with fungal entomopathogens, making them endophytic. Their mode of action against insects appears to be due to antibiosis or feeding deterrence. Res...

The ever increasing movement of viruses around the world poses a major threat to plants growing in cultivated and natural ecosystems. Both generalist and specialist viruses move via trade in plants and plant products. Their potential to damage cultivated plants is well understood, but little attention has been given to the threat such viruses pose to plant biodiversity. To address this, we stud...