Discontinuous increases in the net accumulation of cellular phospholipid were previously observed during the synchronous growth of Rhodopseudomonas sphaeroides (Lueking, D. R., Fraley, R. T., and Kaplan, S. (1978) J. Biol. Chem. 253, 451-457). This pattern is shown to result from large increases in the rate of phospholipid synthesis, occurring just prior to cell division. The absence of detecta...

The possibility of a relationship between intracytoplasmic membranes and deoxyribonucleic acid (DNA) in Escherichia coli O111a(1) has been investigated. To facilitate this investigation, a simple enzymatic assay for the amount of internal membrane present in a culture was developed. This assay was then used to show that the appearance of intracytoplasmic membranes is accompanied by an increase ...

CD70 and CD80 are co-stimulatory molecules which belong to the tumor necrosis factor family and the B7 family respectively. When they are co-expressed by gene-modified TS/A tumor cells, they provide an efficient protective and long-lasting T-dependent antitumor response. We first showed that when CD70 and CD80 were delivered in the tumor environment by gene-modified fibroblasts, but were not ex...

OBJECTIVE To identify the role of Interleukin-l Beta(IL-1β) in patients undergoing intracytoplasmic sperm injection. METHODS The quasi-experimental study was conducted at an infertility clinic in Islamabad from June 2010 to August 2011, and comprised couples opting for intracytoplasmic sperm injection. Down regulation of ovaries was followed by calculated stimulation, ovulation induction, ooc...

BACKGROUND The intracytoplasmic sperm injection procedure ending with total fertilization failure is very distressfull event for both the clinician and the patient. OBJECTIVE The aim of this study was to identify independent factors which could be used to identify total fertilization failure before the day of intracytoplasmic sperm injection. MATERIALS AND METHODS This was a retrospective s...

The aim of this prospective randomized study was to determine if the use of intracytoplasmic morphologically selected sperm injection (IMSI) is associated with gender incidence. Couples who underwent IVF-preimplantation genetic screening (PGS) cycles, as a result of advanced maternal age, were randomly allocated into two groups: intracytoplasmic sperm injection (ICSI; n=80) or intracytoplasmic ...