Although pyridoxal phosphate is known to inhibit gelation of purified hemoglobin S, antisickling activity has never been demonstrated for intact erythrocytes. We incubated washed erythrocytes at 37 degrees C either in buffer alone, or with added pyridoxal phosphate or pyridoxal, washed these cells, suspended them in untreated buffer, and compared the percent modified hemoglobin, the oxygen affi...

OBJECTIVE To examine effects of exercise in the heat and fluid intake on erythrocyte sickling and neutrophil activation in carriers of sickle cell trait (HbAS). DESIGN, SETTING, AND PARTICIPANTS Six African American men (2 HbAS; 42% HbS, 4 HbAA; 20.7 +/- 0.8 years; 87.4 +/- 9.6 kg) participated in 2 randomized sessions (separate days) each consisting of 45 minutes of brisk walking (treadmill)...

S ICKLE cell anemia is a congenital chronic hemolytic type of anemia characterized hematologically by the development of oat-shaped and sickle-shaped erythrocytes. Other cellular abnormalities which are due to excessive blood de-’ struction and active blood formation are also seen in blood smears. Six to IO per cent of Negroes possess the sickle trait 2p 3; their red blood cells have the capaci...

Hemolysis contributes to the pathology associated with sickle cell disease. However, the mechanism of hemolysis or relative contribution of sickling due to hemoglobin (Hb) polymerization vs. oxidative damage remains unknown. Earlier studies aimed at deciphering the relative importance of these two mechanisms have been complicated by the fact that sickle red cells (SS) have already been affected...

Recent findings that hemoglobin S gelation and sickling are pH-dependent and also influence oxygen affinity suggested that the red cells containing this hemoglobin variant might show an abnormal Bohr effect. We therefore studied the effects of pH variation on the in vitro oxygen affinity of whole blood from persons with sickle cell anemia (SS) and normal donors (at 37 degrees C and constant car...

Recent findings that hemoglobin S gelation was greatly increased only between blood and sickling are pH-dependent and also pH 7.4 and 7.2 (cell pH 7.2 and 7.0. a shift influence oxygen affinity suggested that that strongly affects gelation). with L log the red cells containing this hemoglobin p50/ L pH -0.92 to -0.99 (normal variant might show an abnormal Bohr 0.42 to 0.46). Thus a drop in SS b...

To test the antisickling activity of pyridoxal, we compared the oxygen affinity and the percent sickling at low PO2 of untreated erythrocytes with values for cells from the same blood sample incubated with pyridoxal, glyceraldehyde, or pyridoxine. Pyridoxal increased oxygen affinity much more than glyceraldehyde. 20 mM pyridoxal and glyceraldehyde had equivalent antisickling activity. At PO2 le...

Human reticulocytes are capable of synthesizing membrane lipids from 14C-glycerol de novo. In both sickle and nonsickle reticulocytes the majority of 14C-glycerol was incorporated into phospholipids, primarily phosphatidylserine and phosphatidylcholine. Incorporation into sphingomyelin was minimal. The most abundant neutral lipid synthesized was triglyceride. In the absence of sickling, the rat...

OBJECTIVE The objective of this study was to assess the prevalence, gender differences, and time trends of Sickle Cell Trait in the Southern Suburb of Beirut, Lebanon, as well as to highlight the importance of screening for Sickle Cell Trait carriers in this population. Another objective was to describe a new screening technique for Sickle Cell Trait carriers. METHODS This was a retrospective...

BW 12C, a substituted benzaldehyde, stabilises haemoglobin in the oxy-conformation and has attracted interest as an anti-sickling agent. The drug causes a left shift in the oxygen saturation curve and may induce tumour anoxia and enhance the effect of cytotoxic drugs. During clinical trials in patients with cancer abnormal bands on haemoglobin electrophoretic strips were observed. This made cor...