Antibiotic resistance genes are ubiquitous in the environment. However, only a fraction of them are mobile and able to spread to pathogenic bacteria. Until now, studying the mobility of antibiotic resistance genes in environmental resistomes has been challenging due to inadequate sensitivity and difficulties in contig assembly of metagenome based methods. We developed a new cost and labor effic...

We have developed a novel double Amplification Refractory Mutation System (double ARMS) using a highly polymorphic region 5' to the human delta-globin gene as a model system. The double ARMS approach involves using two allele-specific ARMS primers simultaneously during DNA amplification by the polymerase chain reaction (PCR). The resulting system is highly sensitive and more specific than singl...

The polymerase chain reaction (PCR) has been extensively used for the mutation of DNA sequences (1–5,7,8). Many variations of the protocol have been implemented, but most involve a first step in which PCR is used to modify the molecule, and a second step in which the new sequence is subcloned into a vector. A new method, named inverse PCR mutagenesis (IPCRM) has been shown to be an efficient an...

Prediction programs for general PCR (or random PCR) products had been developed [3, 2]. These programs had been experimentally shown to be highly accurate in its prediction [2]. On the other hand, random PCR have been demonstrating that it is very useful as an elementary technology of genome profiling [1], in which random PCR products (a set of DNA fragments extracted from various parts of geno...

BACKGROUND Factor VIII intron 22 inversions (Inv22) cause 40%-45% of severe cases of hemophilia A in all human populations. Currently, Inv22 can be analyzed either by Southern blotting or by rapid long-distance-PCR-based approaches. We describe an alternative method using inverse-PCR (I-PCR). METHODS I-PCR involved 3 steps: (a) BclI restriction; (b) self-ligation of restriction fragments, pro...