Genome-wide Association Study Related to Semen Traits Based on Gene-set Enrichment Analysis in Holstein Bulls

To prevent distribution of recessive alleles in dairy herds all bulls used for AI (Artificial Insemination) haveto be tested. In this study 26 blood and 4 semen samples were supplied from Iranian Holstein bulls used forAI. Genomic DNA was extracted from 100 μl of blood and 200 μl of semen. Samples were tested by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR...

Heat stress has negative effects on pregnancy rates of lactating dairy cattle. There are genetic differences in tolerance to heat stress; Bos taurus indicus (B. t. indicus) cattle and embryos are more thermotolerant than Bos taurus taurus (B. t. taurus). In the present study, the effects of sire and sire breed on conception and embryonic/fetal loss rates of lactating Holstein cows during the Br...

The sperm-specific phospholipase C zeta (PLCz) is a candidate sperm-borne oocyte-activating factor that triggers a characteristic series of physiological stimuli via cytoplasmic Ca(2+) oscillations during fertilization. The molecular mechanisms involved in the regulation of PLCz gene expression remain largely unknown. To explore the genetic variations in the 5'-flanking region of the PLCz gene ...

Pure Holsteins (n = 380) were compared to Normande/Holstein crossbreds (n = 245), Montbeliarde/Holstein crossbreds (n = 494), and Scandinavian Red/Holstein crossbreds (n = 328) for 305-d milk, fat, and protein production during first lactation. Scandinavian Red was a mixture of Swedish Red and Norwegian Red. Cows were housed at 7 commercial dairies in California and calved from June 2002 to Jan...

The Single Nucleotide Polymorphisms (SNP) Discovery on Transcriptome of Pure Sistani and Cross-Breeding Holstein, Simmental Monte Billiard Bulls

Canadian and United States evaluations of Holstein bulls were compared for longevity and somatic cell score. A total of 13 236 bulls was evaluated for longevity with 462 bulls having evaluations in both countries, and 10 945 bulls evaluated for somatic cell score with 564 bulls having evaluations in both countries. Multiple across country evaluation procedures were used to estimate the genetic ...

abcg2 (atp binding cassette subfamily g member 2) gene, located on chromosome 6 encodes the abcg2 protein that transports various xenobiotics, cytostatic drugs across the plasma membrane as well as cholesterol into milk. a single nucleotide change (a/c) in base 86 of exon 14 is capable of encoding a substitution of tyrosine with serine in the abcg2 gene and increase milk yield while decreasing ...

We studied sequence variations in the regulatory region of the bovine growth hormone receptor gene. A polymerase chain reaction (PCR)-based method for detecting AluI, AccI, and StuI restriction fragment length polymorphisms (RFLPs) in the 5' flanking region of the bovine growth hormone receptor gene was developed and tested for association with milk-related traits in Holstein bulls. Allele freq...