The LDH isoenzyme composition of 12 platelet preparations was determined by electrophoresis. The mean (+/- SD) percentages of LDH-1, LDH-2, LDH-3, LDH-4, and LDH-5 were 16.6 +/- 1.7, 30.1 +/- 1.0, 34.2 +/- 1.3, 18.2 +/- 1.3, and 0.9 +/- 1.1, respectively. In comparison with previous data, these data show identical ranking of the prevalence of each isoenzyme but significantly different percentag...

We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis a...

The accuracy of our lactate dehydrogenase (EC 1.1.1.27) isoenzyme procedure, based on electrophoresis in thin-layer agarose, has previously been assessed (Clin. Chem. 22: 1995, 1976). We now show that the method is similarly accurate and precise for the specific measurements of lactate dehydrogenase isoenzyme-1 and -2. We have also determined the lactate dehydrogenase isoenzyme pattern in perip...

A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 ALP3, respec-tively) in bovine serum treated with protease neuraminidase (PN-treatment), although there were concerns intestinal isoenzyme (ALP5) often overlapped ALP3 human neuraminidase. Because ALP5 was separated from alone (P-treat...

Martin-Sanchez, J., and Garcia-Fernandez, P. 1999. Theileria annulata: Genetic characterization of Spanish isolates by isoenzyme electrophoresis and random amplified polymorphic DNA. Experimental Parasitology 92, 57–63. Isoenzyme electrophoresis and RAPD techniques were used to study the genetic polymorphism of different Spanish isolates of Theileria annulata in the schizont and piroplasm stage...

Metastable creatine kinase MM isoenzyme was isolated and partially purified from homogenates of myocardium and skeletal muscle by gradient elution on carboxymethyl cellulose. This variant isoenzyme migrated between the MM and MB isoenzymes on agarose electrophoresis, accounted for 3.5% of the total creatine kinase activity in each tissue, was not a macromolecule, and had stable electrophoretic ...

Extracts of adult schistosomes collected from Hippopotamus amphibius in the Kruger National Park gave alpha naphthyl acetate isoenzyme patterns after polyacrylamide gel electrophoresis that did not correspond with those of either Schistosoma mansoni or S. rodhaini.

Histological examination of supraclavicular lymph node tissue obtained at biopsy from a 63-year-old man disclosed metastatic small-cell carcinoma. On admission and for four days subsequently, total lactate dehydrogenase (LD; EC 1.1.1.27) activity in serum was 6.5 times normal; studies of LD isoenzyme showed persistently increased LD-1, with LD-1 greater than LD-2. Isoenzyme electrophoresis of t...

We assessed the clinical efficacy of a thin-layer agarose electrophoresis assay for lactate dehydrogenase isoenzyme estimation in the diagnosis of acute myocardial infarction. From a population of 228 patients admitted to the Coronary Care Unit with suspected acute myocardial infarction, all 101 positive cases (confirmed by clinical presentation and electrocardiographic changes) were correctly ...

We have adapted to a microcentrifugal analyzer an immunoinhibition assay for measuring the activity of creatine kinase MB by using an inhibitory antibody for the M monomer. The method actually measures half the MB activity, but results are not multiplied by two because atypical isoenzymes of creatine kinase, including BB, IgG-BB, and the isoenzyme derived from mitochondria, are also detected, i...