In this paper, data are presented which demonstrate that adenylate kinase and creatine kinase are oncodevelopmental enzymes in the rat prostate. The Dunning tumor (dorsal rat prostate) was used as a model system; four sublines of the tumor (R3327-H, R3327-AT, MAT Lu, and MAT LyLu) were studied. The tumor lines were maintained as solid tumors in syngeneic rats (Copenhagen) and as monolayers in t...

Detailed procedures are described for the separation and isolation of the isoenzymes of ATP-creatine transphosphorylase (creatine phosphokinase) from autopsy samples of muscle and brain tissue of five terminal dystrophic males (x-linked, Duchenne). These direct isolation studies have led to the remarkable observation that three isoenzymes are present in the atrophied muscle tissue (characterize...

The X-linked enzyme, glucose-6-phosphate dehydrogenase (G-6-PD) was used as a cellular marker to study the clonal characteristics of human atherosclerotic lesions from females heterozygous for G-6-PD isoenzymes. Portions of uninvolved aortic wall contained both isoenzyme types (A and B), and their isoehzyme patterns were used to establish criteria for polyclonal lesions. Portions of uterine lei...

Serum adenylate kinase (AK) activity was measured in cerebral vascular diseases and the AK isoenzyme pattern was studied in the patients suffering from cerebral infarction, cerebral hemorrhage, myocardial infarction, and polymyositis. There was a significant difference in AK activity between the control subjects and patients with acute cerebral infarction. Serum AK activity showed a peak on the...

NAD-specific glutamate dehydrogenase [L-glutamate: NAD+ oxidoreductase (deaminating) EC 1.4.1.2] from Medicago sativa constitutes organ-specific patterns of isoenzymes. The isoenzyme-patterns of seeds (GDH-I) and roots (GDH-II) were purified 1520-fold and 92-fold, respectively. All isoenzymes of both patterns remain stable throughout the purification procedures. Isoenzyme a7, the only isoenzyme...

I measured total creatine kinase (CK; EC 2.7.3.2) activity and isoenzyme pattern in normal and neoplastic tissues. CK activity was detected in all of them examined. In various tumors it was greater than, less than, or the same as that in normal tissue, no clear correlation being seen between total activity and growth rate or degree of differentiation. In several cases, there was a greater propo...