RNA splicing is the process of forming mature mRNA, which an essential phase necessary for gene expression and controls many aspects cell proliferation, survival, differentiation. Abnormal gene-splicing events are closely related to development tumors, generation oncogenic isoform in can promote tumor progression. As a main tumor-specific variants, alternative (AS) progression by increasing pro...

background: it is now well-demonstrated that histone demethylases play an important role in developmental controls, cell-fate decisions, and a variety of diseases such as cancer. lysine-specific demethylase 5d (kdm5d) is a male-specific histone demethylase that specifically demethylates di- and tri-methyl h3k4 at the start site of active genes. in this light, the aim of this study was to invest...

Background: It is now well-demonstrated that histone demethylases play an important role in developmental controls, cell-fate decisions, and a variety of diseases such as cancer. Lysine-specific demethylase 5D (KDM5D) is a male-specific histone demethylase that specifically demethylates di- and tri-methyl H3K4 at the start site of active genes. In this light, the aim of this study was to invest...

The p70 ribosomal S6 kinase (S6K) family is a group of highly conserved kinases in eukaryotes that regulates cell growth, proliferation, and stress response via modulating protein synthesis biogenesis. S6Ks are downstream effectors the Target Rapamycin (TOR) pathway, which connects nutrient energy signaling to growth homeostasis, under normal conditions. plant S6K includes two isoforms, S6K1 S6...

vascular endothelial growth factor (vegf-a) is one of the most important regulatory factors in pathological and physiological angiogenesis. alternative splicing is a complicated molecular process in vegf-a gene expression which adds complexity to vegf-a biology. among all vegf-a exons, alternative splicing of exon 8 is the key determinant of isoform switching from pro-angiogenic vegf-xxx to ant...

Viral transcriptomes that are determined using first- and second-generation sequencing techniques incomplete. Due to the short read length, these methods inefficient or fail distinguish between transcript isoforms, polycistronic RNAs, transcriptional overlaps readthroughs. Additionally, approaches insensitive for identification of splice start sites (TSSs) and, in most cases, end (TESs), especi...

Abstract Objective In this study, we applied two long-read sequencing (LRS) approaches, including single-molecule real-time and nanopore-based methods to investigate the time-lapse transcriptome patterns of host gene expression as a response Vaccinia virus infection. Transcriptomes determined using short-read approaches are incomplete because these platforms inefficient or fail distinguish betw...

In this study, we investigated the cardioprotective effects of resveratrol. Rats were intraperitoneally administered with resveratrol (25 mg/kg bw) or vehicle (ethanol 10%) for 7 days and their heart subjected to ischemia/reperfusion injury. Isolated hearts were langendorff perfused, left ventricular functions as heart rate and developed pressure, as well as, heart antioxidant status were deter...