Background: Infertility problem affects 20-30% of young couples. One of the known causes of spermatogenesis disorder is chemotherapy in patients with cancer which its side effect may last up to the end of the life. The aim of the present study is to investigate the effect of GnRH on viability of ovarian follicles defect produced by anticancer drug. Materials and Methods: In the present study ad...

BACKGROUND Ovarian tissue cryopreservation is an emerging fertility preservation option, and culturing follicles isolated from this tissue to obtain mature gametes may ultimately be the best solution for patients for whom transplantation is contraindicated. It is unclear, however, how patient-specific variables (including age, weight and menstrual cycle stage) impact follicle growth and quality...

background: many attempts have done to improve cryopreservation of mammalian ovaries using simple, economical and efficient technique “vitrification”. objective: the aim of the present study was to compare the mouse ovaries cryopreservation by direct cover vitrification (dcv) using different concentrations of ethylene glycol (eg) with conventional vitrification methods (cv). materials and metho...

BACKGROUND Cryopreservation of isolated follicles may be a potential option to restore fertility in young women with cancer, because it can prevent the risks of cancer transmission. Several freezing protocols are available, including slow-rate freezing, open-pulled straws vitrification (OPS) and solid-surface vitrification (SSV, a new freezing technique). The purpose of our study was to investi...

STUDY QUESTION Do the ovarian follicles of children and adolescents differ in their morphology and in vitro growth potential from those of adults? SUMMARY ANSWER Pre-pubertal ovaries contained a high proportion of morphologically abnormal non-growing follicles, and follicles showed reduced capacity for in vitro growth. WHAT IS KNOWN ALREADY The pre-pubertal ovary is known to contain follicl...

The maturation and developmental competence of the oocyte is acquired during folliculogenesis. It is still unclear whether follicle size is associated with the levels of transcript and protein encoding molecules contributing to the fertilization ability of the porcine oocyte. Follicles were dissected from porcine ovaries after slaughter and classified as small (< 3 mm), medium (3-5 mm) or large...

Background: With all the recent advances in cancer treatments, many young cancer patients find themselves facing the prospect of losing their fertility after aggressive chemotherapy or radiotherapy. Cryopreservation of ovarian cortical tissue has emerged as a potential option to restore fertility in these young women. Materials and Methods: Because autotransplantation of cryopreserved ovarian c...