extraction of intact quality dna from plant tissues, especially those rich in secondary metabolites, is often challenging. literally, hundreds of different dna isolation protocols from various plant species have been published over the last decades. although many commercial dna isolation kits are convenient and designed to be safe, their cost and availability cause limitations in small molecula...

four halotolerant fungal isolates originating from the saltwater lake urmia in iran were selected during a screening program for salt resistance and α-amylase activity. the isolates were identified based on sequencing the its region and a part of the β-tubulin gene, as penicillium chrysogenum (isolate u1; cbs 132820), fusarium incarnatum (isolate u2; cbs 132821), and penicillium polonicum (isol...

abstract background and aims: to identify and isolation of viruses a susceptible cell is need which can be primary or line. the use of primary cells is laborious, time consuming and expensive, while the use of cell lines is much easier and faster. irkhbk cell line was originated from primary cells of bovine kidney by continuous passage. the aim of this study was to introduce the irkhbk cell lin...

traumatic brain injury (tbi) is one the most common cause of disability between young adults. these people have been socially isolated during post-surgery traumatic brain injury. in this study we investigated the effect of social isolation on the structural deficits after tbi in the young rats. young rats were divided into control group with social interaction, sham group with social isolation ...

In this paper, optimal design of hybrid low damping base isolation and magnetorheological (MR) damper has been studied. Optimal hybrid base isolation system has been designed to minimize the maximum base drift of low damping base isolation system where for solving the optimization problem, genetic algorithm (GA) has been used. In design procedure the maximum acceleration of the structure has ...

aspergillus spp. are ubiquitous saprophytic fungi that cause a variety of diseases, ranging from hypersensitivity reactions to flulike pneumonia and life-threatening invasive aspergillosis. as the lung is the primary site of initial infection with airborne conidia, we investigated the innate immune responses of bronchial epithelial cells against different forms of aspergillus spp.

the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...