Antibody opsonins from cystic fibrosis (CF) patients were investigated using nonmucoid and mucoid lipopolysaccharide (LPS) immunotype 1 Pseudomonas aeruginosa as bacterial ligands and PMN phagocytes. CF sera were compared to normal sera, polyvalent PA LPS hyperimmune globulin, and isotype switch variant monoclonal antibodies (MAbs) specific for type 1 PA LPS. Sera from PA-infected CF patients (...

Molecular analyses of murine humoral immune responses have been largely limited to responses induced by soluble proteins, polysaccharides, and hapten conjugates thereof (1-15) . However, in nature the mouse rarely encounters an antigenic structure in association with such chemically simple forms, but rather in association with a viral particle, bacterium, or parasite . How such differences in t...

A careful analysis of the immune response to immunization of amyloid precursor protein/transgenic (APP/Tg) mice with beta-amyloid (Abeta) may provide insights into why a subset of the patients in a clinical trial receiving Abeta-immunotherapy developed encephalomyelitis. Characterization of isotypic immune responses have been reported in different APP/Tg models. In these studies the relative ra...

Tonsillar lymphocytes from an otherwise healthy nonthrombocytopenic male child were fused with the lymphoblastoid cell line GM 4672. Twenty of 472 (4%) hybridomas had antiplatelet reactivity detected using intact platelets in an enzyme-linked immunosorbent assay. One hybridoma (STO 171) reacted to platelet glycoprotein IIb (integrin alpha IIb) as determined by radioimmunoprecipitation and immun...

The first half of this thesis develops an eigenspace approach to computing the basisindependent isotypic decomposition of a vector in a representation of a finite group. The approach takes advantage of well-chosen diagonalizable linear transformations to compute isotypic projections through a series of eigenspace projections, and at its heart is an efficient eigenspace projection method built a...

High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or neonatal inbred mice. Anti-idiotypic antibodies were induced at the same time. The average affinity of the mouse antibodies (Ka approximately equal to 10(7) M-1) is similar to that of rabbit anti-IgD an...

Relative dimensions of isotypic components of N–th order tensor representations of the symmetric group on n letters give a Plancherel– type measure on the space of Young diagrams with n cells and at most N rows. It was conjectured by G. Olshanski that dimensions of isotypic components of tensor representations of finite symmetric groups, after appropriate normalization, converge to a constant w...