The metabolism of propranolol was examined by using microsomes from Dark Agouti rats known as a poor-metabolizer animal model for debrisoquine 4-hydroxylation and Wistar rats. Propranolol 4- and 5-hydroxylations followed biphasic Michaelis-Menten kinetics, and 7-hydroxylation and N-desisopropylation were monophasic in both strains. The kinetic studies showed that the Vmax for propranolol 7-hydr...

HUMAN INTESTINAL ALKALINE PHOSPHATASE. Tissue expression and serum levels. ISBN 91-7174-674-9 ISSN 0346-6612 New series No 342 Ulla Domar, Department of Medical Biochemistry and Biophysics, University of Umeå, S-90187 Umeå, Sweden. Human alkaline phosphatase (ALP) comprises four isozymes, viz liver/bone/ kidney or tissue unspecific (AP), intestinal (LAP), placental (PLAP) and germ cell or PLAP-...

Administration of streptozotocin produces a diabetic condition in mice characterized by a specific decrease in amylase synthesis in the pancreas as well as a substantial reduction in amylase mRNA concentration. We have studied this effect in mice of the congenic strains C3H.AmyYBR and C3H.AmyCE with multiple active copies of the pancreatic amylase structural gene. When mice of these strains are...

Populus cathayana Rehd., a dioecious tree species, is widely distributed in the northern, central and southwestern regions of China. In poplars, Melampsora larici-populina Kleb. is mainly responsible for rust disease, which is considered to be the world's most important disease of poplars. Yet, little is known about sex-related responses to rust disease in poplars. The purpose of this work was ...

Isozymes of several different classes of enzymes in partially purified protein extracts of five strains of Schizophyllum commune, isogenic except for genes controlling sexual morphogenesis, were separated on polyacrylamide gel by disc electrophoresis. After staining, isozyme patterns were compared on the bases of the presence or absence, electrophoretic mobility (R(f) values), and relative acti...

observed also with many tumor-associated antigens, such as a-fehoprotein and carcinoembryonic antigen (3). Al though the phenotypes, including isozyme pattern, in the transplantable humors are known ho be fairly stable through generations of transplantation (1, 17, 50), in certain in stances some phenotypes in Imansplanhablehepahomas alter gradually or suddenly along with increased growth male ...

The enzyme level profiles of some regulatory enzymes and the isozyme patterns of some marker enzymes in bovine adult specialized, adult ordinary and fetal ordinary heart muscles were examined in order to biochemically characterize specialized heart muscle. The activities of hexokinase, phosphofructokinase and glucose-6-phosphate dehydrogenase in adult specialized heart muscle were significantly...

Lactate dehydrogenase isozyme 5 from rabbit skeletal muscle is activated by citrate, cis-aconitate, isocitrate, alpha-ketoglutarate, succinate, fumarate, malate, aspartate, and glutamate. In the presence of these activators the shape of the pyruvate saturation curve is changed from sigmoid to hyperbolic. Lactate dehydrogenase isozyme 1 from rabbit heart gives a hyperbolic pyruvate saturation cu...