Differential expression of isozymes enables fish to tolerate temperature fluctuations in their environment. The present study explores the modulation lactate dehydrogenase (LDH) and cytoplasmic malate (sMDH) isozyme heart, muscle, brain, liver, gill, kidney juvenile Horabagrus brachysoma after 30 days acclimation at 26, 31, 33, 36°C. LDH sMDH zymography were performed using native polyacrylamid...

Phosphofructokinase from rabbit brain consists of hybrids of the A, B, and C isozymes. Phosphofructokinase C was isolated from a purified mixture of such hybrids in a 2-step procedure. In the first step, phosphofructokinase B was removed by chromatography on DEAE-Sephadex. In the second step, subunits of phosphofructokinases A and C were separated by dissociation at pH 5.0 followed by chromatog...

Na,K-ATPase plays a crucial role in cellular ion homeostasis and is the pharmacological receptor for digitalis in man. Nine different human Na,K-ATPase isozymes, composed of 3 alpha and beta isoforms, were expressed in Xenopus oocytes and were analyzed for their transport and pharmacological properties. According to ouabain binding and K(+)-activated pump current measurements, all human isozyme...

Esterases have recurrently been implicated in insecticide resistance in Helicoverpa armigera but little is known about the underlying molecular mechanisms. We used a baculovirus system to express 14 of 30 full-length esterase genes so far identified from midgut cDNA libraries of this species. All 14 produced esterase isozymes after native PAGE and the isozymes for seven of them migrated to two ...

The age-associated alteration in expression levels of carboxylesterases (CESs) can affect both intestinal and hepatic first-pass metabolism after oral administration of xenobiotic esters such as prodrugs. In this study, the age-related expression of CES isozymes and hydrolase activities were simultaneously investigated in liver, jejunum, and ileum from 8-, 46-, and 90-week-old rats. Rat liver e...

Two isozymes of the Na,K-ATPase are made in the brain and retina. Antisera have been produced that contain antibodies specific for each of the catalytic subunits of the two isozymes, alpha and alpha(+) (Sweadner & Gilkeson, 1985a). To determine which classes of cells express which form of the Na,K-ATPase, and whether cells can express both forms, the antisera were used to stain sections of the ...

BACKGROUND We have previously reported that several selective protein kinase C (PKC) inhibitors, including procyanidin B-2, promote hair epithelial cell growth and stimulate anagen induction. OBJECTIVES We discuss the hypothesis that the hair-growing activity of procyanidin B-2 is related to its downregulation or inhibition of translocation of PKC isozymes in hair epithelial cells. METHODS ...

A number of xenobiotics are known to exert their porphyrinogenic effects in rodents and chick embryos through mechanism-based inactivation of certain cytochrome P450 (P450) isozymes. To facilitate the extrapolation of results from test animals to humans, we have assessed the ability of three prototype porphyrinogenic compounds-namely, 3,5-diethoxycarbonyl-1,4-dihydro-2,6-dimethyl-4-ethylpyridin...

The liver is the most active organ engaged in S-adenosyl methionine metabolism . S-Adenosylmethionine synthetase isozymes (EC 2.5.1 .6) were studied in normal, fetal, and pathological livers including hepatoma to correlate their activities with developmental stages and pathophysiologi cal states of the liver. Three isozymes of S-adenosylme thionine synthetase, namely low, intermediate, and high...