In this study, phylogenetic analysis of some fungal species distributed in West Kazakhstan was performed based on ITS sequences. All the mushroom samples were collected from different regions and brought to laboratory. Total genomic DNA extracted using a GeneMark commercial kit. ITS1 ITS4 primers used for amplification region PCR analyses. The resulting sequences then edited BioEdit FinchTV. Fo...

Mutual relationship between endophytic fungi and some gramineuos plants is one of the recently known host-microbe interactions. Endophytic fungi are classified in class Ascomycetes, order Hypocreales, tribe Balansieae and mostly genus Neotyphodium. They are transferred into the progeny via maternal stock and have many benificial effects to their host plants, such as resistance to pests and dise...

Abstract Endophytic fungi are a group of microorganisms colonizing the plant tissue without exposing hazardous effects to their host. Higher plants have endophytic microbes that produce bioactive compounds or secondary metabolites. The aim study was isolate and identify from leaves, stems bark Styrax sumatrana . Samples were collected Huta Gurgur Village, Dolok Sanggul District, Humbang Hasundu...

Apple fruit rot disease is one of the major apple diseases in Indonesia. It has been caused by several species pathogenic within genus Colletotrichum. This research aims to identify pathogen causing Pujon, East Java. Suspected fungal isolates were identified based on morphological (macroscopic and microscopic) molecular characteristics. Based observation, five as Colletotrichum spp. These have ...

O presente estudo teve por objetivo realizar a identificação dos fungos encontrados na própolis produzido Apis mellifera L. da Baía do Iguape, Brasil. Para tanto, foram utilizadas técnicas morfológicas, bioquímicas e moleculares, sendo averiguado o perfil de restrição gerado espaçador interno transcrito (ITS1 ITS4). tamanho produtos PCR foi analisado quanto ao obtidos com endonuclease (HhaI, Ha...

Enophytic fungi were isolated from plum (Prunus domestica) leaves, identified with ITS1 and ITS4 primers, and their antagonistic activity was tested against Monilinia fructicola, which causes brown rot, blossom blight, and twig blight of stone fruits, and Colletotrichum gloeosporioides, which causes anthracnose on a variety of fruit crops. The production of antifungal compounds was determined ...

Validated protocols for DNA purification and PCR amplification are reported for detection of Phytophthora cactorum in diseased strawberry plants. To remove PCR inhibitors, necrotic strawberry tissues were soaked in 5% alconox solution for >12 h before DNA extraction, and the extracted genomic DNA was embedded in an agarose gel chamber and subjected to electrophoresis. The purified DNA was ampli...

Rice blast disease, caused by the fungus Pyricularia oryzae, is one of the most devastating diseases of rice. Understanding pathogenic variation and molecular characterization of P. oryzae is one of the most efficient ways to manage the disease. Studies on identification, characterization and pathogenic variation of P. oryzae were conducted in the laboratory and screen-house at Sokoine Universi...