Correct interactions with extracellular matrix are essential to human pluripotent stem cells (hPSC) to maintain their pluripotent self-renewal capacity during in vitro culture. hPSCs secrete laminin 511/521, one of the most important functional basement membrane components, and they can be maintained on human laminin 511 and 521 in defined culture conditions. However, large-scale production of ...

are placed at the bottom of the jar, one of the usual oxygen indicators (for example, alkali^6' glucose-methylene-blue mixture) placed aloflj? with the cultures, and the jar sealed. After jj to 12 hours, according to the size of the jar an" the amount and quality of iron filings, the colour of the indicator is discharged and co1*^ plete anaerobiosis obtained. Approximately " grammes of iron fil...

AIMS To investigate the effect of Notch2 and Notch3 on cell proliferation and apoptosis of two trophoblast cell lines, BeWo and JAR. METHODS Notch2 and Notch3 expression in BeWo and JAR cells was upregulated or downregulated using lentivirus-mediated overexpression or RNA interference. The effect of Notch2 and Notch3 on cell proliferation was assessed by the CCK-8 assay. The effect of Notch2 ...

Empirical evidence suggests infants can make inferences about uncertain future events using probabilistic data (Denison & Xu, 2010; Teglas et al., 2007). However, it is unclear if infants are using information about proportions to make these decisions. Infants were presented with population jars that contained large distributions of objects, and were tasked with deciding which of the two jars w...

Copying the whole of a library is one of the major types of reuse in software development. In Java, a single library archive file often contains other libraries it depends on, but users of the library hardly know about such inner libraries. Since reusing libraries is a black-box method, developers may combine some libraries without knowing that those libraries contain the same library inside in...

Growth of anaerobic bacteria in the AnaeroPack (Mitsubishi Gas Chemical America, Inc., New York, N.Y.) anaerobic atmosphere generation systems, both the AnaeroPack jar and pouch and the AnaeroPack in a GasPak jar were considered equivalent to or better than growth obtained in the corresponding GasPak jar or pouch system (Becton Dickinson Microbiology Systems, Cockeysville, Md.) for 89 (86%) of ...

At the initial phase of embryo implantation, the trophoblast of the blastocyst has to establish contact to the free surface of the uterine epithelial cells (UECs) and to adhere there. Adhesion to an apical plasma membrane is a very unusual phenomenon, and mechanisms involved are poorty understood. Determination of binding forces using atomic force spectroscopy demonstrated that formation of str...

We explored the cytotoxic effects of nanobacteria (NB) and nanohydroxyapatites (nHAPs) against human choriocarcinoma cells (JAR) and the mechanisms of action underlying their cytotoxicity. JAR cells were co-cultured with NB and nHAPs for 48 h, and ultrastructural changes were more readily induced by NB than nHAPs. Autophagy in the plasma of JAR cells were observed in the NB group. The rate of a...