The purpose of this study is to investigate the performance and three-dimensional behavior of the flow in a mixed flow pump and the way cavitation phenomenon is affected by different parameters such as fluid temperature, pump speed and flow rate. Computational fluid dynamic software FLUENT 6.3 was utilized to simulate the whole flow field of the pump. RNG k-ε model combined with standard wall f...

In excitable tissues, the activity of the plasmalemmal sodium/potassium ATPase (Na/K pump) is vital for the maintenance of normal electrical activity and ion gradients. In cardiac muscle, the transsarcolemmal sodium (Na) gradient established by the Na/K activity is essential not only for generating the rapid upstroke of the action potential but also for driving a number of ion exchange and tran...

The Na+/K+ pump in rat hepatocytes is stimulated in response to Ca2+-mobilizing hormones such as [arginine]vasopressin (AVP), angiotensin II and adrenaline, as well as tumour promoters such as 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA). The ability of these agents to increase cellular contents of diacylglycerol and activate protein kinase C may be necessary to observe this response...

The Na and K dependence of the Na/K pump was measured in skin fibroblasts from patients with cystic fibrosis and age/sex-matched controls. Under basal conditions, there was no difference between control and cystic fibrosis cells in protein per cell, intracellular Na and K content, or Na/K pump activity (measured as ouabain-sensitive 86Rb uptake). There was no difference in the Na dependence of ...

The activity of the Na+-K+-pump is intricately linked to the maintenance of vascular tone. Here we demonstrate that insulin-like growth factor I (IGF-I) increases Na+-K+-pump activity in the vascular smooth muscle cell (VSMC) clone A7r5 in a time- and dose-dependent manner. This stimulatory effect of IGF-I was prevented by the tyrosine kinase inhibitor genistein (5 μM) and by the specific phosp...

For a long period of time, design and manufacturing technology of high flow rated vertically suspended pumps (VSPs) which have an extensive applications in many industries such as water and wastewater, mining, petrochemical and oil and gas industries, used to be imported from European countries. For the first time in Iran's pump industry, with the support of Ministry of Petrochemical[ah1]  and ...

The effect of diabetes on sarcolemmal Na(+)-K(+) pump function is important for our understanding of heart disease associated with diabetes and design of its treatment. We induced diabetes characterized by hyperglycemia but no other major metabolic disturbances in rabbits. Ventricular myocytes isolated from diabetic rabbits and controls were voltage clamped and internally perfused with the whol...

Angiotensin II (Ang II) inhibits the cardiac sarcolemmal Na(+)-K(+) pump via protein kinase (PK)C-dependent activation of NADPH oxidase. We examined whether this is mediated by oxidative modification of the pump subunits. We detected glutathionylation of beta(1), but not alpha(1), subunits in rabbit ventricular myocytes at baseline. beta(1) Subunit glutathionylation was increased by peroxynitri...

The interaction of extracellular Na (Na(o)), K (K(o)), and strophanthidin with the Na-K pump of the human red blood cell has been investigated. Inhibition by submaximal concentrations of strophanthidin rapidly reaches a level which does not increase further over a relatively long period of time. Under these circumstances, it is possible to apply a steady-state kinetic analysis to the interactio...

In virtually all mammalian cells, the traditional roles of the Na/K pump include establishing and maintaining transcellular gradients of Na and K and modulating steady-state gradients of H and Ca. By regulating intracellular Na, [Na]i, the Na/K pump also makes a major contribution to cellular volume control, although some aspects of this modulation of osmolarity are not completely understood (A...