BACKGROUND: BACKGROUND: Enterotoxigenic Escherichia coli (ETEC) is the main causative agents of neonatal calf diarrhea. Passive transfer of antibodies that is derived from the dams of the calves through their colostrum plays an important role in disease prevention. OBJECTIVES: To determine the presence of natural specific antibodies against K99 antigen in dam’s serum and colostrum as well as in...

To evaluate the effect of widely used parenteral vaccination of dams against neonatal colibacillosis, the virulence factors of the intestinal Escherichia coli flora, namely, O serogroup, enterotoxin(s) produced (heat labile, porcine heat stable, and murine heat stable) and adhesins (K88, K99, and 987P antigens) of 149 piglets from different herds in Sweden were investigated. Three categories we...

Twenty-four pregnant cows were vaccinated intramuscularly with K99 extract from enterotoxigenic Escherichia coli and inactivated rotavirus as follows: six cows were injected with 2 ml of oil-adjuvanted vaccine; six cows were injected with 0.5 ml of oil-adjuvanted vaccine; six cows were injected with 4 ml of aluminum hydroxide-adjuvanted vaccine twice with a four-week interval; and six cows were...

Passive protection of neonatal piglets against fatal enteric colibacillosis was achieved with powder preparations of specific antibodies against K88, K99, and 987P fimbrial adhesins of enterotoxigenic Escherichia coli. The antibody powders were obtained by spray drying the water-soluble protein fraction of egg yolks from immunized hens after the lipid components were precipitated with an aqueou...

USP25m is the muscle isoform of the deubiquitinating (DUB) enzyme USP25. Similarly to most DUBs, data on USP25 regulation and substrate recognition is scarce. In silico analysis predicted three ubiquitin binding domains (UBDs) at the N-terminus: one ubiquitin-associated domain (UBA) and two ubiquitin-interacting motifs (UIMs), whereas no clear structural homology at the extended C-terminal regi...

A total of 205 Escherichia coli isolates from 88 diarrheal weanling (4- to 10-week-old) pigs from 59 farms were tested by slide agglutination for K88, K99, F41, and 987P antigens. K88 antigen was detected in 61% of the isolates representing 60% of the pigs and 56% of the farms. K88 antigen was associated with serogroup O149 and 91% of the K88+ isolates. K99, F41, and 987P were not detected. Of ...