A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h. The...

Keratinase is a specific proteolytic enzyme that degrades keratin. has wide applications in global industries. Bacillus sp. MD24 been reported to produce keratinase by solid-state fermentation method using chicken feathers as sole substrate. Furthermore, production could be elevated adding biomass waste (sugarcane bagasse, tofu waste, or rice husk) feathers. However, the ratio of addition was u...

Abstract Poultry industry is expanding rapidly and producing million tons of feather waste annually. Massive production keratinaceous byproducts in the form industrial wastes throughout world necessitates its justified utilization. Chemical treatment keratin proclaimed as an eco-destructive approach by various researchers since it generates secondary pollutants. Keratinase released a variety mi...

The present review deals with fungal keratinases including that of dermatophytes. Bacterial keratinases were also included. Temperature and substrate relationship keratinase production has also been discussed. Keratin degradation and industrial involvement of keratinase producing fungi is also reviewed.

Abstract Keratinase is a robust enzyme that produced in the presence of keratin substrates. This has been recognized for its applications waste management, leather and detergent industries. Our group isolated potential keratinase producing strain Bacillus aerius NSMk2 from poultry dump soil, hide dehairing stain removal have studied. Considering commercial applicability keratinase, present stud...

A newly isolated bacterium identified as Bacillus safensis based on biochemical tests and 16S rRNA analysis and its mutant variant created by exposure to ultraviolet radiation at 254 nm were investigated for keratinolytic activity. The wild-type strain produced 35.4-50.4 U/mL keratinase over a period of 120 h, while the mutant one yielded 64.4-108.5 U/mL keratinase for the same period of 120 h....

Two experiments were conducted to investigate the in vitro ability of keratinase to hydrolyze soybean glycinin and β-conglycinin and to evaluate the in vivo effects of keratinase when included in corn-soybean diets with different levels of crude protein and fed to nursery pigs. In experiment 1, a saturated keratinase solution (1 ml) was added to two blank controls of either glycinin or β-congly...

The effect of onion and garlic extracts on fungal growth and keratinolytic activity was studied in Trichophyton mentagrophytes as one of the major etiologic agents of human and animal dermatophytosis in Iran and other parts of the world. In order to find out the best keratinase producer for further steps, culture conditions for 30 strains of T. mentagrophytes isolated from human dermatophytosis...

Keratinase producing microorganisms are being increasingly utilized for degradation and recycling of poultry feather waste. Two native strains BF11 (Bacillus subtilis) and BF21 (Bacillus cereus) degrading keratin completely were characterized. The native strains produced more than 10 KU/mL of enzyme. Strain improvement resulted in isolation of MBF11 and MBF21 from BF11 and BF21 isolates, respec...