Every year, the size of human population grows; with it, need for agricultural products increases. This leads to an increment in volume waste, including hard-to-degrade keratin-rich ones, such as feathers. Currently, most agro-industrial complex protein by-products are utilized by incineration, landfilling, and chemical hydrolysis. Such methods do not meet modern trends development a sustainabl...

BACKGROUND Hair is composed mainly of keratin protein and a small amount of lipid. Protein hydrolysates, in particular those with low molecular weight distribution have been known to protect hair against chemical and environmental damage. Many types of protein hydrolysates from plants and animals have been used in hair and personal care such as keratin hydrolysates obtained from nails, horns an...

Feathers are the most prevalent agricultural waste generated by chicken farms, polluting environment and wasting protein resources as a result of accumulation large amounts feathers. Therefore, keratinase-producing microorganisms represent promising potential technique for degradation feather waste. Streptomyces netropsis A-ICA Bacillus subtilis ALICA, previously isolated from rhizosphere deser...

Abstract Keratinases can specifically degrade keratins, which widely exist in hair, horns, claws and human skin. There is a great interest developing keratinase to manage keratin waste generated by the poultry industry reusing products agriculture, medical treatment feed industries. Degradation of more environmentally friendly sustainable compared with chemical physical methods. However, wild-t...

Poultry feathers consist mainly of the protein keratin, which is rich in β-pleated sheets and consequently resistant to proteolysis. Although many keratinases have been identified, the reasons for their substrate specificity towards β-keratin remain unclear due to difficulties in preparing a soluble feather keratin substrate for use in activity assays. In the present study, we overexpressed Gal...

The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. In the next step, the synthetic DNA was inserted into the pUC57 vector and then sub-cloned in the ...

A distinctive isolate was discovered and visually recognized as a member of the genus Didymella during routine examination Coelomycetes isolated from diverse fruit juices. Based on sequencing internal transcribed spacer (ITS), fungus identified keratinophila since it showed 100% identity to type strain. The strain thrived produced keratinase collagenase enzymes by hydrolyzing native chicken fea...