Susceptibility to mouse plasmacytomagenesis is a complex genetic trait controlled by several Pctr loci (Pctr1, Pctr2, etc). Congenic strain analysis narrowed the genetic interval surrounding the Pctr2 locus, and genes identified in the interval were sequenced from susceptible BALB/c and resistant DBA/2 mice. Frap (FKBP12 rapamycin-associated protein, mTOR, RAFT) was the only gene differing in a...

Objectives. The total antioxidant capacity (TAC) of a sample can be measured with a ferric reducing antioxidant power (FRAP) assay. There are commercially available kits for FRAP assays, however they are more expensive than in-house kits. We aimed to evaluate a FRAP direct measurement method under our laboratory conditions using a microplate reader and establish reference values to use in futur...

background: previous study by the authors showed that a-tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (pok) after release of 24-h unilateral ureteral obstruction (uuo). this study is a supplementary investigation on the effects of a-tocopherol combined with an antagonist of angiotensin-ii type-1 (at1) receptor on renal dysfunc...

3 Parameter Estimation 7 3.1 Model of FRAP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.2 FRAP simulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.3 FRAP experiments and Latrunculin A . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.4 Boff and Soff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

BACKGROUND Previous study by the authors showed that a-tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (POK) after release of 24-h unilateral ureteral obstruction (UUO). This study is a supplementary investigation on the effects of a-tocopherol combined with an antagonist of angiotensin-II type-1 (AT1) receptor on renal dysfunct...

Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery after Photobleaching (FRAP) are probably the most widely used methods to determine diffusion coefficients in biological samples. However, it has already been noted in the past that the two methods do not necessarily provide the same results. This has typically been explained by the different spatial range over which the two m...

OBJECTIVE Curcumin has anti-inflammatory and antioxidative properties. The objective of this study was to investigate the therapeutic effects of curcumin on functional disturbances, oxidative stress, and leukocyte infiltration induced by renal ischemia/reperfusion (I/R). MATERIALS AND METHODS Animals were randomly divided into 9 groups. The groups with 24-h reperfusion consisted of sham-24h, ...

Fluorescence redistribution after photobleaching (FRAP) has received increasing attention ever since it was first introduced into cell biological research. The method was developed in the 1970s, when its biological application mainly focused on the mobility of fluorescently labelled constituents of the cell membrane. The development of confocal scanning microscopy in the 1980s facilitated accur...

Seasonal discrepancy in the antioxidant potential from bark of Nothapodytes nimmoniana was evaluated using two different methods (DPPH and FRAP) and were related to total phenolic content as well as flavonoid content. Higher yield of phenolic and flavonoid were observed during summer (May) similarly, the antioxidant activities (DPPH & FRAP) also were high. The antioxidant activities were strong...

Background: Previous study by the authors showed that a-tocopherol prevents oxidative stress but would not improve depressed excretory variables in post-obstructed kidney (POK) after release of 24-h unilateral ureteral obstruction (UUO). This study is a supplementary investigation on the effects of a-tocopherol combined with an antagonist of angiotensin-II type-1 (AT1) receptor on renal dysfunc...