نتایج جستجو برای: labeled nitrogen

تعداد نتایج: 192583  

Journal: :The Journal of biological chemistry 1990
M F Roberts B S Choi D E Robertson S Lesage

Turnover of the nitrogen moiety from free amino acid pools in two thermophilic methanogens, Methanobacterium thermautotrophicum delta H and Methanococcus thermolithotrophicus SN1, has been monitored with 15N NMR spectroscopy. In cells growing exponentially on 15NH4Cl, glutamate was the major soluble 15N-labeled species in both organisms. When the Mb. thermoautotrophicum cells were harvested, wa...

Journal: :Journal of proteomics 2015
Keyun Wang Yongjin J Zhou Hongwei Liu Kai Cheng Jiawei Mao Fangjun Wang Wujun Liu Mingliang Ye Zongbao K Zhao Hanfa Zou

UNLABELLED Protein methylation catalyzed by SAM-dependent methyltransferase represents a major PTM involved in many important biological processes. Because methylation can occur on nitrogen, oxygen and sulfur centers and multiple methylation states exist on the nitrogen centers, methylproteome remains poorly documented. Here we present the methylation by isotope labeled SAM (MILS) strategy for ...

2012
Nadezhda E Shchepina Viktor V Avrorin Gennady A Badun Nikolay A Bumagin Scott B Lewis Sergey N Shurov

UNLABELLED BACKGROUND The nuclear-chemical method brought unique opportunity for synthesis of unknown and hardly available organic compounds. Presence of tritium labeling allows one-step preparation of radioactive markers for the investigation of chemical and biological processes. METHODS The ion-molecular reactions of nucleogenic phenyl cations with 4-picoline have been carried out. The p...

Journal: :Journal of nutritional science and vitaminology 1993
S M Domene A C de Oliveira

This study evaluated the digestibility of leguminous protein labeled with 15N, by using nitrogen balance and quantitation of fecal endogenous nitrogen (FEN), determined by isotopic dilution, in order to correct apparent values. Seeds of common beans, cowpea and common pea labeled with 1.000 atoms% of 15N-excess were used as protein sources in diets for 60 male Wistar rats, during a 6-day assay....

Journal: :American journal of physiology. Endocrinology and metabolism 2010
Juan C Marini Inka Cajo Didelija Leticia Castillo Brendan Lee

Although glutamine is considered the main precursor for citrulline synthesis, the current literature does not differentiate between the contribution of glutamine carbon skeleton vs. nonspecific nitrogen (i.e., ammonia) and carbon derived from glutamine oxidation. To elucidate the role of glutamine and nonspecific nitrogen in the synthesis of citrulline, l-[2-(15)N]- and l-[5-(15)N]glutamine and...

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