Signal sequences function in protein targeting to and translocation across the endoplasmic reticulum membrane. To investigate the structural requirements for signal sequence function, chimeras of the Escherichia coli LamB signal peptide and prolactin were prepared. The LamB signal peptide was chosen by virtue of the extensive biophysical and biological characterization of its activity. In vitro...

The appearance of newly induced LamB protein at the cell surface of Escherichia coli was followed topographically by immuno-electron microscopy. LamB protein was induced in E. coli wild-type or lac-lamB cells for a short period of time (4 to 6 min), such that the overall level of LamB protein in induced cells was at least twofold higher than that in uninduced cells. Antibodies bound to LamB pro...

a four- year-old moghanian breed ewe was admitted to the large animal veterinary clinic, university of tabriz due to dystocia. during obstetrical examination, it was revealed that the cervix has been closed firmly and vagina protruded about 5 cm through the vulva (vaginal prolaps). based on clinical findings and for saving the dam and lamb(s), the cesarean section was done. a normal alive femal...

The present study was conducted to evaluate the effect of parenteral supplementation of cobalt, copper and iron in late pregnancy on ewe hematology parameters and lamb vigour. Twenty ewes were allocated to one of two groups (n=10). In the test group, on day 120 ofpregnancy, cobalt, copper and iron were injected at a dose of 0.1 mL/kg BW (Fercobsang, France, cobalt gluconate 5 mg/100 mL, copper ...

Data of 5,390 Merino lambs born from 1986 to 2007 (approximately 6.9 generations) were used to derive genetic parameters and trends for age-specific and overall lamb survival on the underlying scale, as well as for lamb birth weight, using Gibbs sampling. The majority of lambs were descended from lines that were divergently selected for the ability of ewes to rear multiples. The line selected i...

This paper deals with the study of the spectral components of Lamb waves generated in thin aluminium plate using pulsed Nd-YAG laser and detected using a He-Ne Laser Heterodyne interferometer. Laser generation of Lamb waves leads to simultaneous generation of various frequency component as well as multiple Lamb wave modes. The Lamb wave signals are successfully and comprehensively analyzed usin...

In this study 71 head of conceiving ewe and their progeny (91 head) from the flock of Lori-Bakhtiari sheep (360 head), in Sholi Station were randomly selected to determine the relationship between the immunoglobulin (IgG) concentration in the ewe's serum and colostrum, and lamb's serum. Ewe blood sample, colostrum of ewe and blood of the lamb was taken 2 weeks before parturition, 1 to 8 hours a...

To investigate the contribution of LamB in Klebsiella pneumoniae antimicrobial resistance, we determined the MICs of various antibiotics and the frequency of mutation to increased cefoxitin or meropenem resistance of the strains CSUB10S (expressing only OmpK36), CSUB10R (lacking OmpK35 and OmpK36), and their derived isogenic insertion-duplication mutants deficient in LamB. Expression of LamB wa...

BACKGROUND Prion protein (PrP) alleles associated with scrapie susceptibility persist in many sheep populations even with high frequencies despite centuries of selection against them. This suggests that scrapie susceptibility alleles have a pleiotropic effect or are associated with fitness or other traits that have been subject to selection. METHODOLOGY/PRINCIPAL FINDINGS We genotyped all lam...

Among the various techniques available, ultrasonic Lamb waves offer a convenient method of examining composite materials. Since the Lamb wave velocity depends on the elastic properties of a material, an effective tool exists to evaluate composites by measuring the velocity of these waves. Lamb waves can propagate over long distances and are sensitive to the desired in-plane elastic properties o...