Purpose: To evaluate the efficacy and reliability of a low-energy femtosecond laser with a high repetition rate for construction of deep anterior donor corneal lamellae. Methods: This was a prospective laboratory investigation. Twenty-five human corneal buttons were femtosecond laser cut to create thick anterior lamellae (diameter, 10mm; thickness, 500μm). The laser cuts were made using an LDV ...

Electron microscope studies were made on various tunicate oocytes at different stages of growth and development. Both the inner and outer lamellae of the perforated nuclear envelope demonstrate considerable blebbing activity. The blebs of the inner lamella detach into the nucleoplasm where they undergo a special type of fusion process resulting in the formation of numerous, usually single, diff...

Annulate lamellae (AL) have been observed many times over the years on electron micrographs of rapidly dividing cells, but little is known about these unusual organelles consisting stacked sheets endoplasmic reticulum-derived membranes with nuclear pore complexes (NPCs). Evidence growing for a role AL in viral infection. early life cycles hepatitis C virus (HCV) and, more recently, severe acute...

The interlamellar biomechanical properties of stromal collagen are relatively unknown, yet may be highly significant with respect to wound healing and the efficacy of certain keratorefractive surgical procedures. Interlamellar adhesive strength was measured as the tearing force required to separate corneal lamellae at a 50% stromal depth in 16 human eyebank corneas. The mean value for the centr...

(1) Chloroplast lamellae, like many other membranes, are composed of 90-A subunits. (2) The way in which the 90-A units associate with one another determines the quantasome structures of the membrane. There appear to be four 90-A subunits per quantasome in the most highly organized arrays. In at least some cases, the way in which the subunits bind one to another is under environmental control. ...

Observations by transmission electron microscopy show that lamellae of clinoenstatite are present in diopside grains of the Alpe Arami garnet lherzolite of the Swiss Alps. The simplest interpretation of the orientation, crystallography, and microstructures of the lamellae and the phase relationships in this system is that the lamellae originally exsolved as the high-pressure C-centered form of ...

Extract prepared from activated Xenopus eggs is capable of reconstituting nuclei from added DNA or chromatin. We have incubated such extract in the absence of DNA and found that numerous flattened membrane cisternae containing densely spaced pore complexes (annulate lamellae) formed de novo. By electron and immunofluorescence microscopy employing a pore complex-specific antibody we followed the...

Amoebic gill disease (AGD) is the most important parasitic disease of Atlantic salmon industry inAustralia. Atlantic salmon (Salmo salar) experimentally infected with Neoparamoeba sp. apparently showedAGD gross signs on the gill and an amoebic-associated gill pathology. Physico-chemical factors of waterduring the experiment were monitored regularly and were approximately constant (temperature: ...