an agrobacterium-mediated transient gene expression assay was carried out in alfalfa (medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of foot and mouth disease virus called vp1. the plant leaves were transformed via agroinfiltration procedure. the presence of the foreign gene and its expression in transformed plants were evaluated by polymerase chain ...

oral supplementation with 50, 100 and 150 ?g/ml calcium chloride to the fifth stadium larvae of csr2xcsr4 biovoltine hybrid silkworm, b. mori on fat body glycogen, protein, and total lipids and haemolymph trehalose and protein have been studied. one of the four normal feeds per day was substituted with treated leaves fed to silkworms at fifth instar larvae. the controls were fed with the leaves...

Oral Supplementation with 50, 100 and 150 ?g/ml calcium chloride to the fifth stadium larvae of CSR2xCSR4 biovoltine hybrid silkworm, B. mori on fat body glycogen, protein, and total lipids and haemolymph trehalose and protein have been studied. One of the four normal feeds per day was substituted with treated leaves fed to silkworms at fifth instar larvae. The controls were fed with the leaves...

To investigate sepal/petal/lip formation in Oncidium Gower Ramsey, three paleoAPETALA3 genes, O. Gower Ramsey MADS box gene5 (OMADS5; clade 1), OMADS3 (clade 2), and OMADS9 (clade 3), and one PISTILLATA gene, OMADS8, were characterized. The OMADS8 and OMADS3 mRNAs were expressed in all four floral organs as well as in vegetative leaves. The OMADS9 mRNA was only strongly detected in petals and l...

The primary purpose of this study was to examine the effects virus infection in Phalaenopsis cultivated across country. RT-PCR analysis used detect two major viruses orchid: CymMV and ORSV. rate vitro plantlets from 45 cultivars 83.6%, potted plants 21 92.5%. For CymMV, single 34.3%, for ORSV, it 11.4%. In case plants, with 57.0%, ORSV alone not detected. double both viruses, accounted 37.9% 35...

Hydrangea (Hydrangea macrophylla) is a well known Al-accumulating plant, showing a high level of aluminum (Al) tolerance and accumulation. Although the physiological mechanisms for detoxification of Al and the roles of Al in blue hydrangea sepals have been reported, the molecular mechanisms of Al tolerance and accumulation are poorly understood in hydrangea. In this study, we conducted a genome...

In Petunia x hybrida 'Fantasy Red', a leucine-rich repeat (LRR) gene referred to as PhLRR, was identified in a flower bud cDNA library. The open reading frame sequence of PhLRR was 1251 bp, encoding a putative 46.2-kDa protein of 416 amino acids. The PhLRR protein showed high similarity to members of polygalacturonase inhibitor proteins (PGIPs), contained 11 conserved LRR domains, and was an ex...

FLOWERING LOCUS T (FT) encodes a systemic signal communicating the perception of long day photoperiod from leaves to the shoot apex to induce the floral transition. Transient expression of FT in the phloem companion cells of rosette leaves for one to several days was previously shown to be sufficient to commit plants to flowering. Here we show that partial commitment results in pleiotropic infl...