We used the RAPD-PCR method for distinguishing the main Old World Leishmania parasites Leishmania tropica, L. major, and L. infantum and applied it to Sauroleishmania species from Iran. Twelve out of 21 tested primers were suitable for identification of these parasites. The Jaccard similarity index was 0.30 for L. tropica and L. infantum as well as for L. major and L. infantum. The similarity c...

Leishmania (L.) infantum is the causative agent in an endemic focus of canine leishmaniasis in the Mont-Rolland district (Thiès, Senegal). In this area, the transmission cycle is well established and more than 30% of dogs and 20% of humans are seropositive for L. infantum. However, the sand fly species involved in L. infantum transmission cycle are still unknown. Between 2007 and 2010, 3654 san...

The most frequently used diagnostic methods were compared in a longitudinal survey with Leishmania infantum-infected asymptomatic dogs from an area of Italy where leishmaniasis is endemic. In February and March 2005, 845 asymptomatic dogs were tested by an immunofluorescence antibody test (IFAT), a dipstick assay (DS), and an enzyme-linked immunosorbent assay (ELISA) for L. infantum and by IFAT...

Parole chiave Cane, Epidemiologia, kDNA RFLP-PCR, Leishmania infantum, Tipizzazione molecolare. Riassunto Il metodo più frequentemente utilizzato per la classificazione di Leishmania spp. è ancora rappresentato dalla caratterizzazione isoenzimatica sebbene sia un metodo laborioso e in alcuni casi non sufficientemente discriminante. Nel presente studio abbiamo utilizzato una reazione di amplific...

The single gene encoding cyclopropane fatty acid synthetase (CFAS) is present in Leishmania infantum, L. mexicana and L. braziliensis but absent from L. major, a causative agent of cutaneous leishmaniasis. In L. infantum, usually causative agent of visceral leishmaniasis, the CFAS gene is transcribed in both insect (extracellular) and host (intracellular) stages of the parasite life cycle. Tagg...

Fifty dogs from the area around Barcelona, Spain, were evaluated for serologic evidence of exposure to vector-borne pathogens. Dirofilaria immitis, E h r l i c h i a c a n i s, Borrelia burgdorferi, L e i s h m a n i a infantum, Bartonella vinsonii s u b s p e c i e s b e r k h o f f i i , and Rickettsia rickettsii a n t i g e n s were used for testing purposes. Seroreactivity was determined in...

are PCR positive and/or leishmanial antibody positive become infected with L. infantum. Persons with advanced AIDS or other immunosuppressive conditions seemingly would be at greatest risk for VL. In Brazil, legislation requires that all blood for transfusion be tested for T. cruzi ., hepatitis B and C, T. pallidum, human T-cell lymphotropic virus types 1 and 2, and HIV1 and -2. As additional i...