We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.

This paper presents a PC-based thermal cycler with a local-host architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented local-host system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.

As health services move toward universal methicillin-resistant Staphylococcus aureus (MRSA) screening for hospital admissions, the most cost-effective approach is yet to be defined. In this study, one of the largest to date, we evaluated the performance of the BD GeneOhm MRSA assay on the Rotor-Gene 6000 thermal cycler, using samples taken directly from pooled MRSA screens. Results were compare...

Seagrass meadows are threatened by coastal development and global change. In the face of these pressures, molecular techniques such as reverse transcription quantitative real-time PCR (RT-qPCR) have great potential to improve management of these ecosystems by allowing early detection of chronic stress. In RT-qPCR, the expression levels of target genes are estimated on the basis of reference gen...

Pada masa pandemi Covid-19, teknik penggandaan DNA dengan metode Polymerase Chain Reaction (PCR) menjadi gold standard untuk diagnosis virus dan penghitungan sampel klinis. Metode PCR ini diimplementasikan pada alat nama Thermal Cycler atau mesin PCR. Salah satu komponen utama dalam adalah pemanas. Tiga buah pemanas, yakni: Peltier Device 12715, Ceramic Heater PTC 12 VDC, Customized VDC diuji k...

BACKGROUND Nosocomial sepsis (NS) in newborns (NBs) is associated with high mortality rates and low microbial recovery rates. To overcome the latter problem, new techniques in molecular biology are being used. OBJECTIVES To evaluate the diagnostic efficacy of SeptiFast test for the diagnosis of nosocomial sepsis in the newborn. MATERIALS AND METHODS 86 blood specimens of NBs with suspected ...

OBJECTIVES Asthma is a complex multifactorial disease with an obvious genetic predisposition, immunological aberration, and involvement of noxious environmental factors. Polymorphisms of the glutathione-S-transferase (GST) genes are known risk factors for some environmentally-related diseases. In the present study, the hypothesis that polymorphisms in the GSTT1, GSTM1 and GSTP1 genes are associ...