background and objectives: timely diagnosis of leptospirosis is essential for an effective treatment. large diversity of clinical symptoms has led leptospirosis diagnosis difficult. researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are mo...

Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from ma...

results the lipl32 sequences were >94% homologous among the vaccinal and other pathogenic leptospira serovars in genbank. this result indicates the conservation of this gene within the pathogenic leptospires. conclusions the cloned gene in this study may provide a potentially suitable platform for development of a variety of applications such as serological diagnostic tests or recombinant vacci...

The most common presentation of animal leptospirosis is the subclinical and silent chronic form, that can lead to important reproductive disorders. diagnosis this form remains a challenge. aim present study gather critically analyse current information about molecular tools applied diagnosis, particularly infection. Regarding clinical specimens, samples from urinary tract were used (69/102, 67·...

The identification of the subset of outer membrane proteins exposed on the surface of a bacterial cell (the surfaceome) is critical to understanding the interactions of bacteria with their environments and greatly narrows the search for protective antigens of extracellular pathogens. The surfaceome of Leptospira was investigated by biotin labeling of viable leptospires, affinity capture of the ...

BACKGROUND & OBJECTIVES Leptospirosis, a zoonosis with a worldwide distribution is an acute febrile illness caused by spirochaetes of the pathogenic Leptospira interrogans. Microscopic agglutination test (MAT), the reference method for diagnosis was successively done to evaluate the modified ELISA which was developed with the recombinant LipL32 antigen for the detection of anti-leptospiral anti...

Abstract Introduction Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation determine the severity of infection, especially acute stage, allowing early detection f...

The presence of white spots in the kidneys of cattle at slaughter (so-called white-spotted kidneys) can be an indication of infection with Leptospira, a spirochaete of public health concern because it causes zoonotic disease. In this study, 24 kidneys of 180 slaughtered cows (13.3%) showed focal to multifocal white spots at inspection. These kidneys, together with matching urine (n = 18) an...